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. 2023 Oct 5;6(12):e202302148. doi: 10.26508/lsa.202302148

Figure 6. Knockdown of COX-2 represses HIF-1ɑ–dependent glycolytic shift and M1 polarization in THP-1 macrophages.

Figure 6.

(A) EGFP fluorescence of THP-1 macrophages transfected with lentivirus. (B, C) COX-2–silencing THP-1 macrophages were verified by RT–qPCR and Western blot. (Statistical analysis was performed using a t test, **P < 0.01.) (D, E, F, G) HIF-1ɑ expression (D), lactate production (E), and mRNA and protein expressions of TNF-α, IL-1β, and IL-6 (F, G) were decreased in COX-2–silencing THP-1 macrophages, as quantified by RT–qPCR and ELISA. (Statistical analysis was performed using a t test, *P < 0.05, **P < 0.01, and ***P < 0.001).