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SDS-polyacrylamide gel of L1 purification. Lane 1, Novagen perfect protein molecular weight markers; lane 2, boiled cell fraction of BL21(DE3)pLysS containing pUB5832 before induction; lane 3, boiled cell fraction of BL21(DE3)pLysS containing pUB5832 after a 2-h induction with 1 mM IPTG; lane 4, crude protein after French press; lane 5, purified L1. Molecular weights are noted at the left.
