TABLE 2.
Steady-state kinetic constants of recombinant L1 and other metallo-β-lactamases isolated from S. maltophilia in cacodylate buffer
| Compound | Recombinant L1a
|
Metallo-β-lactamase isolated from S. maltophilia
|
|||
|---|---|---|---|---|---|
| Km (μM) | kcat (s−1) | Km (μM) | kcat (s−1) | Reference | |
| Piperacillin | 38 ± 3 | 440 ± 10 | 20 ± 1 | 140 | 10 |
| Ampicillin | 55 ± 5 | 520 ± 10 | 40 ± 3 | 175 | 10 |
| BRL42715 | 240 ± 20 | 210 ± 10 | 270 ± 10 | 0.55 | 16 |
| Penicillin G | 22 ± 3 | 480 ± 10 | 50 ± 6 | 1,100 | 10 |
| Nitrocefin | 4 ± 1 | 41 ± 1 | 7 ± 1 | 20 | 10 |
a
The buffer used for studies with recombinant L1 was 50 mM sodium cacodylate, pH 7.0, containing 100 μM Zn(II) at 25°C.