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. 2023 Jun 20;15:220–228. doi: 10.1016/j.xjon.2023.05.013

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Antiapoptotic signaling. Immunoblotting showed a significant increase in concentration of anti-apoptotic Bcl-2-associated death promoter (pBAD) in the hypoxia-conditioned extracellular vesicle group (HEV, n = 7) compared with saline control (CON, n = 6). Phospho-B-cell lymphoma 2 (pBcl-2) showed a trend towards an increase in HEV treatment, and B-cell lymphoma 2 (Bcl-2) was significantly decreased with HEV treatment compared to CON. This resulted in a significant increase in the pBcl-2 to BCL-2 ratio in the HEV group. Graphs represent data points with mean with standard deviation. Immunoblotting data are -fold change normalized to average control. Data points greater than 2 standard deviations from the mean are excluded from analysis. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; AT, alpha-tubulin. ∗∗P < .01, ∗∗∗P < .001.