Table 1.
Apoptotic markers
| Marker | HEV (mean ± standard deviation) | P |
|---|---|---|
| Proapoptotic | ||
| AIF | 1.03 ± 0.07 | .69 |
| BAX | 0.93 ± 0.36 | .76 |
| Caspase-3 | 0.82 ± 0.18 | .26 |
| Caspase-9 | 0.82 ± 0.12 | .44 |
| FAK | 0.98 ± 0.13 | .83 |
| FAS | 1.01 ± 0.28 | .94 |
| FOXO1 | 0.97 ± 0.31 | .87 |
| Antiapoptotic | ||
| pAKT | 1.42 ± 0.55 | .10 |
| AKT | 1.08 ± 0.31 | .84 |
| pAKT/AKT ratio | 1.29 ± 0.23 | .04 |
| pBAD | 1.87 ± 0.48 | .005 |
| Bcl-2 | 0.60 ± 0.17 | .006 |
| pBcl-2 | 1.23 ± 0.14 | .12 |
| pBcl-2/Bcl-2 ratio | 2.64 ± 0.49 | <.001 |
| pERK | 2.05 ± 1.19 | .05 |
| ERK | 1.38 ± 0.37 | .008 |
| pERK/ERK ratio | 1.47 ± 0.52 | .18 |
| pFOXO1 | 1.37 ± 0.15 | .04 |
| pFOXO1/FOXO1 ratio | 1.53 ± 0.45 | .02 |
| IL-3 | 1.07 ± 0.06 | .31 |
| PI3K | 1.53 ± 0.23 | .008 |
| pP90RSK | 1.40 ± 0.25 | .37 |
| P90RSK | 1.04 ± 0.28 | .18 |
| pP90RSK/P90RSK ratio | 1.42 ± 0.39 | .92 |
Shown are immunoblotting results for all markers tested in the study. Immunoblotting data are represented as mean fold change in hypoxia-conditioned extracellular vesicle group (HEV, n = 7) with standard deviation (SD) normalized to average control (n = 6). Data points greater than 2 standard deviations from the mean are excluded from analysis. P values less than .05 are shown in bold. HEV, Hypoxia-conditioned extracellular vesicle group; AIF, apoptosis-inducing factor; BAX, bcl-2-like protein 4; FAK, focal adhesion kinase; FAS, Fas cell surface Death receptor; FOXO1, forkhead box O1; pAKT, phospho-protein kinase B; AKT, protein kinase B; pBAD, phospho-Bcl-2-associated death promoter; Bcl-2, B-cell lymphoma 2; pBcl-2, phospho-B-cell lymphoma 2; pERK, phospho-extracellular signal-regulated kinase; ERK, extracellular signal-regulated kinase; pFOXO1, phospho-forkhead box O; IL-3, interleukin 3; PI3K, phosphoinositide 3-kinase; pP90RSK, phospho-p90 ribosomal S6 kinase; P90RSK, p90 ribosomal S6 kinase.