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. 2023 Sep 22;14:1071630. doi: 10.3389/fendo.2023.1071630

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Copyright © 2023 Huang, Wang, Lin, Guan, Liang and Shen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NETs induced by MSU crystals degrade inflammatory factors and alleviate the inflammatory response in gouty arthritis. (A, B) Neutrophils extracted from peripheral blood of patients with gouty arthritis could be induced to form NETs by MSU crystals in vitro. Immunofluorescence (MPO and H3cit positive) (A) and SYTOX Green fluorescence (B) indicated the formation of NETs. C The serum dsDNA detected by ELISA in acute stage and remission stage was tested respectively. The expression of dsDNA increased during emission stage (P=0.008). (D) The expression of serum inflammatory cytokines (IL-1β, MCP-1, TNF, IL-6) in acute stage and remission stage was detected respectively. IL-1β, MCP-1, TNF and IL-6 decreased significantly (P<0.05). (E) The dsDNA is proportional to the decrease of inflammatory cytokines (P<0.05). (F) Neutrophils extracted from patients with gouty arthritis were induced to form NETs by MSU crystals and added to the medium containing IL-1β, MCP-1, TNF and IL-6. The reduction of these inflammatory factors at different time points was analyzed by ELISA.