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. 2023 Sep 20;11:1279446. doi: 10.3389/fbioe.2023.1279446

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Copyright © 2023 Zhang, Lu, Xiao, Hu, Cai, Liu, Liu, Li, Zhou, Qian, Wang, Chen, Zeng and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Synthesis and characterization of Fe₃O₄-Cy nanoparticles. (A) Representative TEM images of hydrophobic Fe₃O₄ nanoparticles, Fe₃O₄-DP-PEG, and Fe₃O₄-Cy, along with corresponding size histograms (B); (C) UV absorption spectra of Fe₃O₄-DP-PEG, Cy5.5-NHS, and Fe₃O₄-Cy; (D) Fluorescence images of Fe₃O₄-DP-PEG, Cy5.5-NHS, and Fe₃O₄-Cy and fluorescence spectra of iron oxide nanoparticles before and after Cy5.5 modification; (E) Plots of R1 and R2 against Fe concentrations of Fe₃O₄-Cy, overlaid with linear fitting curves for extracting longitudinal and transverse relaxivities; (F) Hydrodynamic size of Fe₃O₄-DP-PEG and Fe₃O₄-Cy; (G) Zeta potential of Fe₃O₄-DP-PEG and Fe₃O₄-Cy; (H) Hydrodynamic size changes of Fe₃O₄-Cy incubated in 10% FBS for 72 h.