Figure 2.

Single bacterium proteomics workflow. The cultured E. coli cells were isolated as single or double cells on 96-well plates by FACS; lysed by freeze-and-thaw, and digested before TMT-10plex labeling. Data acquisition was achieved on an Orbitrap Fusion Eclipse mass spectrometer equipped with FAIMS Pro, collecting MS2 spectra for reporter ion based quantification.