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. 2023 Oct 5;32:09636897231202541. doi: 10.1177/09636897231202541

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Isolation and identification of hPDLSCs. (A) hPDLSCs were successfully isolated and cultured, showing long spindle shape under light microscope. Scale bar: 200 μm. (B) Alizarin red S staining revealed a large number of mineralized nodules in hPDLSCs. Scale bar: 200 μm. (C) Oil red O staining revealed a large number of lipids in hPDLSCs. Scale bar: 200 μm. (D) Flow cytometry revealed that hPDLSCs expressed almost no CD34 or CD45 but had positive expressions of CD90, CD105, CD146, and STRO-1. hPDLSCs: human periodontal ligament stem cells.