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. 2023 Oct 5;32:09636897231202541. doi: 10.1177/09636897231202541

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Immunofluorescence analysis of ALP expression in hPDLSCs after coculture with OM and of 1,25(OH)₂VitD₃ ALP was stained with red fluorescence (CoraLite594), actin was stained with green fluorescence (Actin-Tracker Green-488), and nuclei are stained with blue fluorescence (DAPI). Scale bar: 20 μm. ALP: alkaline phosphatase; DAPI: 4′,6-diamidino-2-phenylindole; hPDLSCs: human periodontal ligament stem cells; OM: osteogenic induction medium.