Table 1.
In vitro liquid culture erythroid differentiation media constituents and their working concentrations.
| Component | Manufacturer | Catalog no. | Final concentration | |
|---|---|---|---|---|
| Iscove’s modified Dulbecco’s media (IMDM) GlutaMAX | Gibco | 31980030 | - | 1,2,3 |
| Inactivated group AB Plasma | Dept. of Haematology, Oxford University Hospitals Trust | - | 3% | 1,2,3 |
| FBS | Sigma-Aldrich | 2% | 1,2,3 | |
| Human holo- transferrin | Sigma-Aldrich | T0665 | 200μg/ml / 500μg/ml | 1,2/3 |
| Recombinant human insulin | Sigma-Aldrich | I9278 | 10μg/ml | 1,2,3 |
| Heparin sodium | Sigma-Aldrich | H3149 | 3U/ml | 1,2,3 |
| Recombinant erythropoietin- a | Bio-Rad | OBT1538 | 3U/ml | 1,2,3 |
| SCF | PeproTech | 300–07 | 10ng/ml | 1,2 |
| Penicillin/Streptomycin | Thermo Fisher Scientific | 15140122 | 100U/ml | 1,2,3 |
| Interleukin-3 | PeproTech | 200–03 | lng/ml | 1 |