Table 2.
Example RNP complexing working conditions.
| sgRNA mass (μg) | sgRNA volume (μl) | Cas9 mass (μg) | Cas9 working conc. (μg/ml) | Cas9 volume (μl) |
|---|---|---|---|---|
| 1.6 | 1.6 | 3 | 3 | 1 |
| 3.2 | 3.2 | 6 | 6 | 1 |
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