Table 3.
Flow cytometry antibodies used in this study, grouped according to experimental panel.
| Antibodies | ||||
|---|---|---|---|---|
| Panel | Marker | Fluorophore | Manufacturer | Cat. no. |
| Fetal liver | Ter119 | PE | BioLegend | 116207 |
| Erythroid | CD71 | FITC | BD Phar mingen | 561936 |
| NK1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| B220 | APC-Cy7 | BioLegend | 103224 | |
| CD11b | APC-Cy7 | BioLegend | 101226 | |
| Gr1 | APC/Fire 750 | BioLegend | 108455 | |
| ps6 | c-kit | BV421 | BioLegend | 105827 |
| CD71 | FITC | BD Pharmingen | 561936 | |
| Ter119 | PE | BioLegend | 116207 | |
| pS6 | APC | Cell Signalling Technologies | 4851 | |
| B220 | APC-Cy7 | BioLegend | 103224 | |
| NK1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| Gr1 | APC/Fire 750 | BioLegend | 108455 | |
| CD11b | APC-Cy7 | BioLegend | 101226 | |
| Progenitors | CD48 | PB | BioLegend | 103417 |
| CD41 | BV605 | BioLegend | 133921 | |
| CD34 | FITC | BD Pharmingen | 560238 | |
| CD16/32 | PercyP Cy5.5 | BioLegend | 101323 | |
| c-kit | Biotinylated | BioLegend | 105803 | |
| Streptavidin | PE | BioLegend | 405203 | |
| Sca-1 | PE-Cy7 | BioLegend | 108113 | |
| CD150 | APC | BioLegend 115909 | ||
| B220 | APC-Cy7 | BioLegend | 103224 | |
| NK1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| Gr1 | APC/Fire 750 | BioLegend | 108455 | |
| CD11b | APC-Cy7 | BioLegend | 101226 | |
| Ter119 | APC-Cy7 | BioLegend | 116223 | |
| Myeloid | CD11b | BV421 | BioLegend | 101235 |
| Ter119 | BV510 | BioLegend | 116237 | |
| Ly6C | BV605 | BioLegend | 128035 | |
| F4/80 | AF488 | BioLegend | 123119 | |
| CD71 | PE | BioLegend | 113807 | |
| c-kit | PE-Cy7 | BioLegend | 105813 | |
| Ly6G | PerCPCy5.5 | BioLegend | 127615 | |
| CD115 | APC | BioLegend | 135509 | |
| B220 | APC-Cy7 | BioLegend | 103224 | |
| NK1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| Autophagy | c-kit | BV421 | BioLegend | 105827 |
| Cyto-ID | FITC | Enzo | ||
| Ter119 | PE | BioLegend | 116207 | |
| Sca-1 | PE-Cy7 | BioLegend | 108113 | |
| CD71 | APC | BioLegend | 113819 | |
| B220 | APC-Cy7 | BioLegend | 103224 | |
| NK1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| Gr1 | APC/Fire 750 | BioLegend | 108455 | |
| CD11b | APCCy7 | BioLegend | 101226 | |
| Human | ||||
| pS6 / 4E-BP1 | Hoescht 333258 |
(VL-1) | ||
| p4E-BP1 | PE | Cell Signalling Technologies | 7547 | |
| pS6 | APC | Cell Signalling Technologies | 4851 | |
| Fetal liver - bone marrow chimaera | ||||
| Progenitors | Ter119 | BV421 | BioLegend | 116233 |
| CD16/32 | BV510 | BioLegend | 101333 | |
| CD41 | BV605 | BioLegend | 133921 | |
| CD45.2 biotin | n/a | BioLegend | 109803 | |
| strepdavidin | BUV395 | BD Biosciences | 564176 | |
| Sca-1 | BV785 | BioLegend | 108139 | |
| CD105 | FITC | BioLegend | 120405 | |
| c-kit | PE-Cy7 | BioLegend | 105813 | |
| CD150 | APC | BioLegend | 115909 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| B220 | APC-Cy7 | BioLegend | 103224 | |
| Nk1.1 | APC-Cy7 | BioLegend | 108723 | |
| CD11b | APC-Cy7 | BioLegend | 101226 | |
| Ly6C | APC-Cy7 | BioLegend | 128017 | |
| Erythroid recipient bone marrow | Ter119 | FITC | BioLegend | 116205 |
| CD71 | PE-Cy7 | BioLegend | 113811 | |
| CD44 | APC | BioLegend | 103011 | |
| CD90.2 | APC-Cy7 | BioLegend | 105327 | |
| CD11b | APC-Cy7 | BioLegend | 101226 | |
| B220 | APC-Cy7 | BioLegend | 108455 | |
| Gr1 | APC/Fire 750 | BioLegend | 108455 |