Table 1.

MS coefficients of WT and mutants.

	Stability rank (decreasing)	Average	MD1	MD2	MD3	MD4	MD5
Wildtype (WT)	1	0.0037	0.0029	0.0028	0.0048	0.0037*	0.0047
L167F/P168S/L57F	6	0.0056	0.0055*	0.0069	0.0111	0.0038	0.0045
L167F/F305L/S144A	3	0.0044	0.0029	0.0044*	0.0052	0.0042	0.0047
L167F/F305L/P184S	5	0.0051	0.0049	0.0071	0.0050*	0.0054	0.0026
L167F/F305L/F8L	2	0.0042	0.0046	0.0041*	0.0038	0.0039	0.0083
L167F/F305L/P184S/T21I	4	0.0045	0.0040	0.0044*	0.0052	0.0049	0.0038

MS coefficients were determined for each TTMD replicate, as well as the average MS value was calculated across three different replicates after discarding the highest and lowest values.

The representative replicate (i.e., the closest to the average MS value), is highlighted with a star (*).