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[Preprint]. 2023 Sep 25:2023.09.25.559410. [Version 1] doi: 10.1101/2023.09.25.559410

Fig. 6: Investigating the interactions of AlbAB with AlbC and tRNA.

Fig. 6:

a, Agarose gel shift assays of AlbAB, AlbC, and E. coli total tRNA, as well as combinations thereof. Agarose gel stained for nucleic acids (GelCode Red) (left). The same agarose gel stained for protein (GelCode Blue). b, SDS-PAGE gel of concentrated size-exclusion chromatography void fractions of AlbAB, AlbC, and E. coli total tRNA, as well as combinations thereof. A Superdex 200 Increase column was used. AlbAB filaments exclusively elude in the void while neither AlbC nor E. coli total tRNA does. c, Negative stain TEM micrographs of the AlbAB-containing samples from part b. No samples demonstrate clear accessory binding of AlbC or tRNAs along AlbAB filaments.