| Cell line (human) |
HeLa cell line with inducible Cas9 (cTT20.11) |
Gift from Kara L. McKinley and Iain Cheeseman (McKinley and Cheeseman, 2017) |
N/A |
Parent cell line used for NPM1-tagged reporter cells |
| Cell line (human) |
Lenti-X HEK 293T cells |
Takara Biosciences |
Cat# 632180 |
For lentivirus production. |
| Cell line (human) |
NPM1-mNeonGreen HeLa |
This paper |
N/A |
Tagged at endogenous locus. |
| Cell line (human) |
NPM1-mScarlet HeLa |
This paper |
N/A |
Tagged at endogenous locus. |
| Cell line (human) |
NPM1WT-mScarlet HeLa |
This paper |
N/A |
|
| Cell line (human) |
NPM1mA2-mScarlet HeLa |
This paper |
N/A |
|
| Cell line (human) |
NPM1mB1-mScarlet HeLa |
This paper |
N/A |
|
| Cell line (human) |
NPM1mA3-mScarlet HeLa |
This paper |
N/A |
|
| Cell line (human) |
NPM1mB2-mScarlet HeLa |
This paper |
N/A |
|
| Construct/Plasmid |
GFP-NPM WT |
Gift from Xin Wang, (Wang. W et al., 2005) |
Addgene # 17578 |
Used to obtain sequence of NPM1-WT. |
| Construct/Plasmid |
pX330 |
Gift from Feng Zhang |
Addgene # 42230 |
|
| Construct/Plasmid |
pKLM73 |
Gift from Kara L. McKinley |
|
Donor plasmid with C-terminal mNeonGreen and puromycin resistance cassette |
| Construct/Plasmid |
pKLM110 |
Gift from Kara L. McKinley |
|
Donor plasmid with C-terminal mScarlet and puromycin resistance cassette |
| Construct/Plasmid |
pKLM79 |
Gift from Kara L. McKinley |
|
Lentivirus transfer plasmid with C-terminal mScarlet Fusion tag. SFFV promoter. Used for NPM1 mutant constructs. |
| Construct/Plasmid |
NPM1-mNeonGreen-PuroR donor plasmid |
This paper |
|
|
| Construct/Plasmid |
NPM1-mScarlet-PuroR donor plasmid |
This paper |
|
|
| Construct/Plasmid |
pX330-NPM1 sgRNA |
This paper |
|
Targeting sequence: GCCAGAGATCTTGAATAGCC |
| Construct/Plasmid |
SFFV-NPM1WT-mScarlet |
This paper |
|
|
| Construct/Plasmid |
SFFV-NPM1mA2-mScarlet |
This paper |
|
|
| Construct/Plasmid |
SFFV-NPM1mB1-mScarlet |
This paper |
|
|
| Construct/Plasmid |
SFFV-NPM1mA3-mScarlet |
This paper |
|
|
| Construct/Plasmid |
SFFV-NPM1mB2-mScarlet |
This paper |
|
|
| Software |
μManager |
Edelstein et al., 2014 |
Latest nightly build |
https://micro-manager.org/
|
| Software |
Pycromanager |
Pinkard, H. et al., 2021 |
|
https://github.com/micro-manager/pycro-manager/
|
| Software |
R |
R Core Team, 2021 |
v. 4.1.1 |
https://www.r-project.org/
|
| Software |
Prism |
GraphPad |
v. 8.3.0 |
|
| Software |
FIJI |
Schindelin et al., 2012 |
|
https://imagej.net/software/fiji/
|
| Software |
CellProfiler |
Stirling, D. R. et al., 2021 |
v.4.0.7 |
https://cellprofiler.org/
|
| Oligonucleotide |
5’ETS RNA FISH probe |
Integrated DNA Technologies |
|
Cy3-CGGAGGCCCAACCTCTCCGACGACAGGTCGCCAGAGGACAGCGTGTCAGC |
| Oligonucleotide |
ITS1 RNA FISH probe |
Integrated DNA Technologies |
|
Cy3-CCTCGCCCTCCGGGCTCCGGGCTCCGTTAATGATC |
| Oligonucleotide |
ITS2 RNA FISH probe |
Integrated DNA Technologies |
|
Cy3-CTGCGAGGGAACCCCCAGCCGCGCA |
| Oligonucleotide |
SKIV2L2 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-031902-02-0005 |
|
| Oligonucleotide |
UPF1 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-011763-00-0005 |
|
| Oligonucleotide |
DDX54 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-017128-01-0005 |
|
| Oligonucleotide |
eIF4A3 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-020762-00-0005 |
|
| Oligonucleotide |
NSA2 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-017043-01-0005 |
|
| Oligonucleotide |
MDN1 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-009786-00-0005 |
|
| Oligonucleotide |
RPF2 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-024715-01-0005 |
|
| Oligonucleotide |
GNL2 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-020392-01-0005 |
|
| Oligonucleotide |
PHF5A ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-014987-01-0005 |
|
| Oligonucleotide |
CNOT1 ON-TARGETplus siRNA SMARTPool |
Horizon Discovery |
Cat # L-015369-01-0005 |
|
| Antibody |
α-tubulin Monoclonal antibody (DM1A) |
Invitrogen |
Cat # 62204 |
|
| Antibody |
eIF4A3 polyclonal antibody (Rabbit) |
Proteintech |
Cat 3 17504-1-AP |
|
| Antibody |
UPF1 (D15G6) Rabbit mAb |
Cell Signaling Technology |
Cat # 12040 |
|
| Antibody |
SKIV2L2 polyclonal antibody (Rabbit) |
Novus Biologicals |
Cat # NB100-1574 |
|
| Antibody |
DDX54 polyclonal antibody (Rabbit) |
Proteintech |
Cat # 26894-1-AP |
|
| Antibody |
NOL1 Antibody |
Novus Biologicals |
Cat # NBP1-92192 |
|
| Antibody |
GNL2 Antibody |
Novus Biologicals |
Cat # NBP1-81649 |
|
| Antibody |
GLTSCR2 Polyclonal Antibody |
Proteintech |
Cat # 27353-1-AP |
|
| Antibody |
Goat anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody, Alexa 647 |
Invitrogen |
Cat # A-21235 |
|
| Antibody |
Goat anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, Alexa 647 |
Invitrogen |
Cat # A-21245 |
|
| Antibody |
Anti-mouse IgG, HRP-linked |
Cell Signaling Technology |
Cat # 7076S |
|
| Antibody |
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody |
Millipore |
Cat # A0545-1ML |
|
| Chemical |
Actinomycin D |
Sigma |
Cat # A1410-2MG |
|
| Chemical |
CX-5461 |
SelleckChem |
Cat # S2684 |
|
| Chemical |
Pladienlolide B |
Tocris |
Cat # 6070 |
|
| Chemical |
Hoechst 22242 |
ThermoFisher |
Cat # H3570 |
|
| Chemical/Reagent |
Lipofectamine LTX |
ThermoFisher |
Cat # 15338030 |
|
| Chemical/Reagent |
Lipofectamine 2000 |
ThermoFisher |
Cat # 11668027 |
|
| Chemical/Reagent |
Lipofectamine RNAiMAX |
ThermoFisher |
Cat # 13778075 |
|
