Figure 6. Adaptive immune system is required for CARG-2020 anti-tumor activity.

A. Diagram of study design. TKO mouse ovarian cancer cells were injected i.p. in athymic nude mice (day 0). Mice were randomized into PBS control and CARG-2020 treatment groups on day 6 (n=5). Treatment was administered i.p. at 1×10⁶ PFU per dose for 3 doses (arrows); B. top panel, representative live animal imaging; bottom panel, quantification of tumor burden using mCherry ROI fluorescence area; C. Representative dot plots of peritoneal lavage analyzed for CD11b and CD206; D. Representative dot plots of peritoneal lavage analyzed for CD11b and MCHII; E. Quantification of D. Gating strategy is shown in Supp. Fig. 1. Data are presented as Mean ± SEM and unpaired two-tailed Student t-test was used for statistical analysis.