Figure 7.

Cxcr2 is directly repressed by the glucocorticoid receptor in differentiated 3T3-L1 adipocytes. (A) 3T3-L1 cells were treated with vehicle (control) (white bars) or 100 nM Dex, a synthetic GR agonist, for 3 and 6 hours (grey bars). Gene expression for Cxcr2, Coch, Cemip, Ighm, and Dnase1 were assessed by qPCR. (B) 3T3-L1 adipocytes were pretreated with vehicle (control) (white bars), 100 nM Dex (grey bars), 1 µM RU 486 (slanted black striped bars), 1 µM RU 486 and vehicle (slanted white striped bars) or both 1 µM RU 486 and 100 nM Dex (white dots on black bars). mRNA expression levels were measured by qPCR. (C) 3T3-L1 cells were pretreated for 1 hour with vehicle (control) (white bars), 100 nM Dex (grey bars), 10 µg/mL cycloheximide (slanted black stripes on white bars), or combined cycloheximide followed by 100 nM Dex for 3 hours (slanted black stripes on grey bars). mRNA levels of Cxcr2, Coch, Cemip, Ighm, and Dnase1 were measured using qPCR. All values represent mean ± SD. Data were combined from 3 independent experiments (in duplicate for each experiment) and statistically analyzed using 2-way ANOVA. P-values were based on Tukey's multiple comparison test. * P < .05, ** P < .01, *** P < .001, **** P < .0001.

Abbreviations: GR, glucocorticoid receptors; qPCR, quantitative PCR.