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. Author manuscript; available in PMC: 2024 Oct 3.
Published in final edited form as: Cell Metab. 2023 Oct 3;35(10):1688–1703.e10. doi: 10.1016/j.cmet.2023.09.004

Figure 6. Acod1 blunts TIN ferroptosis through activating Nrf2-mediated antioxidant response.

Figure 6.

(A) Immunoblot of Acod1 and Nrf2 in TINs isolated from E0771 lung metastases in WT and Acod1‒/‒ mice. (B) qRT-PCR of Nrf2 in TINs isolated from E0771 lung metastases in WT and Acod1‒/‒ mice (n=5). (C) qRT-PCR of Gpx4, Gclc and Nqo1 in TINs isolated from E0771 lung metastases in WT and Acod1‒/‒ mice (n=5) treated with vehicle or ML385 (30mg kg‒1, i.p., daily for 7 days). (D-F) The effect of ML385 (10μM) on E0771 TES-primed WT and Acod1‒/‒ BMDNs for measures including lipid peroxidation (BODIPY 581/591 C11), cell death fractions, and viable cell counts (n=5/condition). (G-I) The effect of NK252 on E0771 TES-primed WT and Acod1‒/‒ BMDNs for measures including lipid peroxidation (BODIPY 581/591 C11), cell death fractions, and viable cell counts (n=5/condition). (J-K) GSH and GSSG abundance in E0771 CM-induced WT and Acod1‒/‒ BMDNs treated with DMSO, 0.25mM 4OI or 0.25mM DMI (n=3). Data represent mean ± s.e.m.; ns, not significant, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, unpaired two-tailed Student’s t-test. Experiments were repeated three times with consistent results. See also Figure S6.