Table 1.

Individual results for each tumor xenograft.

	Xenograft cell line	Tumor Volume (mm3)						Sensitivity								Specificity					Dice Coefficient									Maximum surface distance (mm)
		Optical	T1	T2	T1 and T2	PET	MRI+PET	T1	T2		T1 and T2		PET		MRI+PET	T1	T2	T1 and T2	PET	MRI+PET	T1	T2		T1 and T2		PET		MRI+PET		T1	T2		T1 and T2		PET		MRI+PET
Gd+ N=4	GB126	161	122	101	128	185	191	0.74	0.62		0.77		0.87		0.91	0.99	1	0.99	0.91	0.91	0.84	0.76		0.86		0.81		0.83		1.6	1.8		1.6		2.0		2.0
	GB126	86	61	52	63	112	117	0.68	0.59		0.70		0.84		0.89	1	1	1	0.93	0.93	0.80	0.74		0.81		0.73		0.75		1.1	1.1		1.1		2.0		2.0
	GB126	124	69	58	81	151	153	0.53	0.44		0.61		0.90		0.91	0.99	0.99	0.99	0.92	0.92	0.68	0.60		0.74		0.81		0.81		2.6	2.9		2.6		1.8		1.8
	U87	104	89	122	133	211	226	0.76	0.79		0.84		0.93		0.95	0.99	0.94	0.93	0.82	0.80	0.82	0.72		0.74		0.61		0.60		1.2	2.1		2.1		2.3		2.3
Gd+ Tumors Median		114	79	80	105	168	172	0.71	0.61		0.74		0.89		0.91	0.99	0.99	0.99	0.91	0.91	0.81	0.73		0.78		0.77		0.78		1.4	2.0		1.9		2		2
Gd- N=9	GB187	104	0	72	72	161	172	0	0.53		0.53		0.85		0.89	1	0.97	0.97	0.88	0.87	0	0.63		0.63		0.67		0.67		nd	2.7		2.7		2.8		2.8
	GB187	76	0	60	60	87	109	0	0.52		0.52		0.56		0.73	1	0.96	0.96	0.91	0.90	0	0.58		0.58		0.53		0.60		nd	3.2		3.2		3.1		3.1
	GB187	30	0	28	28	29	47	0	0.73		0.73		0.46		0.88	1	0.97	0.97	0.93	0.91	0	0.76		0.76		0.47		0.69		nd	1.2		1.2		2.0		2.0
	GB187	63	0	60	60	69	89	0	0.63		0.63		0.67		0.80	1	0.96	0.96	0.95	0.93	0	0.64		0.64		0.63		0.66		nd	1.8		1.8		2.4		2.4
	U251	64	0	94	94	120	133	0	0.71		0.71		0.82		0.86	1	0.89	0.89	0.84	0.82	0	0.58		0.58		0.57		0.56		nd	2.4		2.4		2.4		2.4
	U251	22	0	8	8	0	8	0	0.24		0.24		0		0.24	1	0.99	0.99	1	0.99	0	0.35		0.35		0		0.35		nd	3.1		3.1		nd		3.1
	GB7	132	0	71	71	0	71	0	0.37		0.37		0		0.37	1	0.97	0.97	1	0.97	0	0.47		0.47		0		0.47		nd	7.6		7.6		nd		7.6
	GB7	131	0	39	39	0	39	0	0.21		0.21		0		0.21	1	0.98	0.98	1	0.98	0	0.32		0.32		0		0.32		nd	8.6		8.6		nd		8.6
	GB94	20	0	57	57	0	57	0	0.35		0.35		0		0.35	1	0.93	0.93	1	0.93	0	0.18		0.18		0		0.18		nd	4.7		4.7		nd		4.7
Gd- Tumors Median		64	0	60	60	29	71	0	0.52		0.52		0.46		0.73	1	0.97	0.97	0.93	0.93	0	0.58		0.58		0.47		0.56		nd	3.1		3.1		2.4		3.1
Overall Median		95	0	60	67	87	110	0	0.56		0.61		0.67		0.87	1	0.97	0.97	0.93	0.92	0	0.62		0.64		0.57		0.63		1.4	2.6		2.5		2.2		2.4

Optical, tdTomato optical images; T1, gadolinium-enhanced t1-weighted MRI; T2, t2-weighted MRI; MRI, combine t1 and t2-weighted MRI; PET, Amino acid PET; MRI+PET, combine t1 and t2-weighted MRI and PET; nd, entire tumor not detected (not included in median calculations for surface distance); Gd+, Gadolinium enhancing tumor (N=4); Gd-, Gadolinium non-enhancing tumor (N=9, PET-detected: N=5, PET-undetected: N=4).