Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, male, C57BL/6) | Sirt2 knockout mice | Dr. Gius Lab | refer to: SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity. | |
| Strain, strain background (Mus musculus, male, C57BL/6) | Sirt2 flox/flox mince | Dr. Gius Lab | refer to: SIRT2 deletion enhances KRAS-induced tumorigenesis in vivo by regulating K147 acetylation status | |
| Strain, strain background (Mus musculus, male, C57BL/6) | Nrf2 knockout mice | the Jackson Laboratory | RRID:IMSR_JAX:017009 | |
| Strain, strain background (Rattus norvegicus domestica, female) | Sprague–Dawley rat | Charles River | ||
| Antibody | Rabbit polyclonal SIRT1 antibody | Sigma | 07–131 | WB (1:1000) |
| Antibody | Rabbit polyclonal SIRT2 antibody | Sigma | S8447 | WB (1:1000) |
| Antibody | Rabbit mAb SIRT3 antibody | Cell Signaling Technology | Rabbit mAb #5490 | WB (1:1000) |
| Antibody | Rabbit polyclonal anti-HPRT antibody | Proteintech | 150-59-1-AP | WB (1:5000) |
| Antibody | Mouse monoclonal anti-GAPDH antibody | Proteintech | 60004–1-Ig | WB (1:10000) |
| Antibody | Rabbit mAb NRF2 antibody | Cell Signaling Technology | Rabbit mAb #20733 | WB (1:1000) |
| Antibody | Rabbit polycloncal NRF2 antibody | Abcam | ab31163 | WB (1:1000) |
| Antibody | HRP-conjugated donkey polyclonal anti-mouse IgG antibody | Jackson ImmunoResearch |
715-035-150 | WB (1:5000) |
| Antibody | HRP-conjugated donkey polyclonal anti-rabbit IgG antibody | Jackson ImmunoResearch |
711-035-152 | WB (1:5000) |
| Antibody | Rabbit Anti beta Actin antibody | Abcam | ab8227 | WB (1:2000) |
| Antibody | RabbTBP antibody | Abcam | ab63766 | WB (1:2000) |
| Antibody | Rabbit mAb SIRT6 antibody | Cell Signaling Technology | Rabbit mAb #12486 | WB (1:1000) |
| Antibody | Mouse Flag-M2 monoclonal antibody | Sigma | F1804 | WB: (1:2000); IP(1:xxx) |
| Antibody | Acetyl Lysine Antibody, Agarose | immunechem | ICP0388-2MG | IP: 1:10 |
| Chemical compound, drug | Propodium Iodine | Sigma | P4170-10MG | |
| Chemical compound, drug | Hoechst 34432 | Life Technology | 62249 | |
| Chemical compound, drug | Cycloheximide | Sigma | 1810 | |
| Chemical compound, drug | Paraformaldehyde | Thermo Fisher Scientific | AC416780250 | |
| Chemical compound, drug | RIPA Buffer | Thermo Fisher Scientific | 89901 | |
| Chemical compound, drug | ProteaseArrest Protease Inhibitor | G-Biosciences | 786–437 | |
| Chemical compound, drug | 10% formalin | Fisher Scientific | FLSF1004 | |
| Chemical compound, drug | BrdU | Sigma | 19–160 | |
| Chemical compound, drug | vitamin B12 | Sigma | V2876 | |
| Chemical compound, drug | FBS | Bio-Techne | S11550 | |
| Chemical compound, drug | penicillin–streptomycin | Cytiva | SV30010 | |
| Chemical compound, drug | Lipofectamine 2000 Transfection Reagent | Invitrogen, Thermo Fisher Scientific | 11668027 | |
| Chemical compound, drug | Dharmafect transfection reagent | Horizon | 2001–03 | |
| Chemical compound, drug | AGK2 | Selleckchem | S7577 | |
| Chemical compound, drug | hydrogen peroxide | Fisher Scientific | H324-500 | |
| Chemical compound, drug | Normal Rabbit IgG | Sigma | 12–370 | |
| Chemical compound, drug | DMSO | Sigma | D4540 | |
| Cell line | H9c2 | ATCC | CRL-1446 | |
| Other | DMEM | Corning | 10-013CV | |
| Commercial assay or kit | RNA-STAT60 | Teltest | Cs-502 | |
| Commercial assay or kit | DNAse I | Ambion | AM2222 | |
| Commercial assay or kit | PerfeCTa SYBR Green FastMix | Quanta | 95074–05 K | |
| Commercial assay or kit | qScript cDNA Synthesis Kit | Quanta | 95047–500 | |
| Commercial assay or kit | SuperSignal West Pico PLUS Chemiluminescent Substrate | Pierce | 34579 | |
| Commercial assay or kit | BCA Protein Assay Kit | Pierce | 23225 | |
| Commercial assay or kit | NE-PER Nuclear and Cytoplasmic Extraction Reagents | Pierce | PI78835 | |
| Commercial assay or kit | Trichrome Stain (Masson) Kit | Sigma | HT15-1KT | |
| Commercial assay or kit | Protein A Agarose | Roche | 11719408001 | |
| Commercial assay or kit | dihydroethidium (DHE) assay | Thermo Fisher Scientific | D11347 | |
| Sequence-based reagent | Rat Sirt2 siRNA | Horizon Discovery | M-082072-01-0005 | siGENOME Rat Sirt2 (361532) siRNA |
| Recombinant DNA reagent | Wildtype SIRT2 plasmid | Dr. Gius Lab | refer to: SIRT2 Maintains Genome Integrity and Suppresses Tumorigenesis through Regulating APC/C Activity | |
| Software, algorithm | GraphPad Prism | GraphPad | Version 9 | |
| Software, algorithm | ImageJ | NIH | 1.53 c |