Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | nosNGT-GAL4 | Bloomington Drosophila Stock Center | Cat: #31777 RRID:BDSC_31777 |
FlyBase symbol: P{Gal4-nos.NGT}40 |
| Genetic reagent (D. melanogaster) | Maternal-tubulin-Gal4 | Bloomington Drosophila Stock Center | Cat: #2318 PMID:23105012 |
FlyBase symbol: P{matα4-GAL-VP16} |
| Genetic reagent (D. melanogaster) | UAS-GCaMP6m | Bloomington Drosophila Stock Center | Cat: #42748 RRID:BDSC_42748 |
FlyBase symbol: w[1118]; P{y[+t7.7] w[+mC]=20XUAS-IVS-GCaMP6m}attP40 |
| Genetic reagent (D. melanogaster) | Gr5a-GAL4 | Bloomington Drosophila Stock Center | Cat: #57592; RRID:BDSC_57592 |
FlyBase symbol: w[*]; P{w[+mC]=Gr5a-GAL4.8.5}6; Dr[1]/TM3, Sb |
| Genetic reagent (D. melanogaster) | UAS-E(z) RNAi | Tsinghua Fly Center | Cat: #2831 | |
| Genetic reagent (D. melanogaster) | UAS-Su(var)3–9 RNAi | Tsinghua Fly Center | Cat: #3558 | |
| Genetic reagent (D. melanogaster) | UAS-Rpd3 RNAi | Tsinghua Fly Center | Cat: #0695 | |
| Genetic reagent (D. melanogaster) | UAS-cad RNAi | Tsinghua Fly Center | Cat: #03,877.N | |
| Antibody | Anti-Histone H3 (di methyl K9) mouse polyclonal |
Abcam | Cat: #ab1220 RRID:AB_449854 |
ChIP-seq 10 μg |
| Antibody | Anti-acetyl histone-h3-k27 rabbit polyclonal |
Abcam | Cat: #ab4729 RRID:AB_2118291 |
ChIP-seq 10 μg |
| Antibody | Anti-Histone H3 (tri methyl K27) mouse polyclonal |
Abcam | Cat: #ab6002 RRID:AB_305237 |
ChIP-seq 10 μg |
| Antibody | Anti-Histone H3 (tri methyl K9) rabbit polyclonal |
Abcam | Cat: #ab8898 RRID:AB_306848 |
ChIP-seq 10 μg |
| Antibody | Anti-Histone H3 antibody rabbit polyclonal |
Abcam | Cat: #ab1791 RRID:AB_302613 |
ChIP-seq 10 μg WB 1:1000 |
| Antibody | Anti-Histone H3 (tri methyl K27) rabbit polyclonal |
Jingjie PTM BioLab | Cat: #PTM-647RM | WB 1:1000 IF 1:200 |
| Antibody | Anti-rabbit IgG, HRP-linked Antibody goat polyclonal |
Jackson ImmunoResearch Laboratories Inc | Cat: #111-035-003 RRID: AB_2313567 |
WB 1:1000 |
| Antibody | Anti-Histone H3 (tri methyl K27) mouse polyclonal |
Jingjie PTM BioLab | Cat: #PTM-5002 | WB 1:1000 IF 1:200 |
| Antibody | Goat anti-mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 goat polyclonal |
Invitrogen | Cat: #a11001 RRID: AB_2534069 |
IF 1:1000 |
| Antibody | Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 goat polyclonal |
Invitrogen | Cat: #a21071 RRID: AB_2535732 |
IF 1:1000 |
| Chemical compound | Fluoroshield with DAPI | Sigma-Aldrich | Cat: #F6057 | 10 μL |
| Chemical compound | EED226 | Selleck | Cat: #S8496 | 5 μM |
| Chemical compound | Chaetocin | Selleck | Cat: #S8068 | 5 μM |
| Chemical compound | A-395 | Sigma-Aldrich | Cat: #SML1923 | 10 μM |
| Commercial assay or kit | Graduated glass capillary | VWR | Cat: #53432-604 | |
| Commercial assay or kit | Triglyceride Quantification Colorimetric/Fluorometric Kit | Nanjing Jiancheng Bioengineering Institute | Cat: #A110-1-1 | |
| Commercial assay or kit | Liver/Muscle glycogen assay kit | Nanjing Jiancheng Bioengineering Institute | Cat: #A043-1-1 | |
| Commercial assay or kit | Trehalose quantification kit | Nanjing Jiancheng Bioengineering Institute | Cat: #A149-1-1 | |
| Commercial assay or kit | MOPS Running Buffer Powder MOPS | GenScript Biotech Corporation | Cat: #M00138 | for Western blotting |
| Commercial assay or kit | ExpressPlus PAGE Gel,10×8, 4–20%, 15 wells | GenScript Biotech Corporation | Cat: #M42015c | for Western blotting |
| Commercial assay or kit | SDS-PAGE Sample Loading Buffer，5× | Biosharp | Cat: #BL502A | for Western blotting |
| Commercial assay or kit | Affinity ECL kit(picogram) | Affinity | Cat: #KF8001 | for Western blotting |
| Commercial assay or kit | Goat Serum | Beyotime | Cat: #C0265 | for Western blotting |
| Chemical compound | Hexanoic acid | macklin | Cat: #H810882 | |
| Chemical compound | Sucrose | Sigma-Aldrich | Cat: #S0389 | |
| Chemical compound | NaCl | Sigma-Aldrich | Cat: #S3014 | |
| Chemical compound | CaCl2 | Sigma-Aldrich | Cat: #C5670 | |
| Chemical compound | MgCl2 | Sigma-Aldrich | Cat: #M4880 | |
| Chemical compound | KCl | Sigma-Aldrich | Cat: #P9541 | |
| Chemical compound | HEPES | Sigma-Aldrich | Cat: #54457 | |
| Chemical compound | NaHCO3 | Sigma-Aldrich | Cat: #S5761 | |
| Chemical compound | NaH2PO4 | Sigma-Aldrich | Cat: #S3139 | |
| Software, algorithm | Fiji/ImageJ | NIH | https://imagej.nih.gov/ij/download.html | |
| Software, algorithm | Rstudio | https://www.rstudio.com/ | ||
| Software, algorithm | GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientificsoftware/prism/ | |
| Software, algorithm | R version 3.6 and 4.0 | Camp Pontanezen – The R Foundation for Statistical Computing | https://www.r-project.org/ | |
| Software, algorithm | Bowtie2 version 2.3.5.1 | https://bowtie-bio.sourceforge.net/bowtie2/index.shtml | ||
| Software, algorithm | samtools version 1.10. | http://www.htslib.org/ | ||
| Software, algorithm | deeptools version 3.3.1 | https://deeptools.readthedocs.io/en/develop/ | ||
| Software, algorithm | IGV version 2.4.13 | https://software.broadinstitute.org/software/igv/download |