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. 2023 Aug 25;9(9):e19315. doi: 10.1016/j.heliyon.2023.e19315

Table 3.

Evaluation of the effect of the flavin reductase as biocatalyst. a.

Entry Catalyst Buffer Yield (%) b
1 Flavin reductase Tris-HCl pH 7 36
2 Flavin reductase No buffer 35
3 Uninduced cell lysate soup Tris-HCl pH 8 23
4 Denaturated flavin reductase No buffer 17
5 BSA No buffer 15
6 No catalyst No buffer 18

3.2.

a

reaction condition: 1a (0.2 mmol), 2a (0.1 mmol) and 100 μg catalyst in 2 ml solvent at room temperature for 24 h.

b

isolated yield.