Table 3.
Evaluation of the effect of the flavin reductase as biocatalyst. a.
| Entry | Catalyst | Buffer | Yield (%) b |
|---|---|---|---|
| 1 | Flavin reductase | Tris-HCl pH 7 | 36 |
| 2 | Flavin reductase | No buffer | 35 |
| 3 | Uninduced cell lysate soup | Tris-HCl pH 8 | 23 |
| 4 | Denaturated flavin reductase | No buffer | 17 |
| 5 | BSA | No buffer | 15 |
| 6 | No catalyst | No buffer | 18 |
reaction condition: 1a (0.2 mmol), 2a (0.1 mmol) and 100 μg catalyst in 2 ml solvent at room temperature for 24 h.
isolated yield.
