Table 1.

Comparison of gliadin extraction conditions: Gliadin extraction ability of different buffer conditions prepared with detergents assessed in wheat flour are measured by OD at 426 nm. Different letters indicate significant differences between treatments. Treatment conditions include [1]: PBS [2], combination of PBS and ethanol [3], combination of 50 mM 2-mercaptoethanol and 2 M guanidine hydrochloride [4], 0.1% EDTA in PBS [5], 0.1% EDTA in ethanol [6], 0.1% Tween 20 in PBS [7], 0.1% Tween 20 in ethanol [8], 0.1% Tween 80 in PBS [9], 0.1% Tween 80 in ethanol [10], 0.1% Triton X-100 in PBS [11], 0.1% Triton X-100 in ethanol. Columns for each condition represents the dilution rates (1:10 and 1:100 for other conditions).

Sample Dilution Extraction Buffer	1:10	1:100
1- only PBS	1.36 ± 0.28 b	0.77 ± 0.26 b,
2- PBS and EtOH	1.21 ± 0.25 b, c	0.69 ± 0.13 b, c
3–50 mM 2-mercaptoethanol and 2 M guanidine hydrochloride in EtOH	2.54 ± 0.08 a	1.59 ± 0.09 a
4–0.1% EDTA in PBS	0.75 ± 0.13 d, e	0.44 ± 0.05 b, c
5–0.1% EDTA in EtOH	0.92 ± 0.13 c, d	0.78 ± 0.12 b
6–0.1% Tween-20 in PBS	0.58 ± 0.06 d, e	0.37 ± 0.10 c
7–0.1% Tween-20 in EtOH	0.57 ± 0.02 d, e	0.53 ± 0.01 b, c
8–0.1% Tween-80 in PBS	0.43 ± 0.09 d, e	0.36 ± 0.10 c
9–0.1% Tween-80 in EtOH	0.70 ± 0.11 d, e	0.60 ± 0.23 b, c
10–0.1% Triton X-100 in PBS	0.37 ± 0.19 e	0.51 ± 0.13 b, c
11–0.1% Triton X-100 in EtOH	0.47 ± 0.04 d, e	0.42 ± 0.02 b, c