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. 2023 Oct 6;14:6259. doi: 10.1038/s41467-023-42029-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Two haplotypes of WH1 are determined by the presence or absence of 180 bp insertion (red bar) in the promoter region. TSS: transcription start site. b The distributions of the two haplotypes of WH1 in relict and non-relict ecotypes. c WH1 negatively regulates UVB resistance. d Survival rates were collected after 4 days recovery. Data are mean ± SD from independent biological replicates (n = 3), and two tailed t-test was used for significance statistics. e The relative mRNA level of WH1 in two haplotypes. Data are mean ± SD from independent biological replicates (n = 3), and two tailed t-test was used for significance statistics. f Schematic diagram of reporters of transient dual-luciferase assay. LUC: Firefly Luciferase; REN: Renilla Luciferase; NOS: NOS terminator. WH1 promoter fragments (1550 bp) were cloned from Yilong-0 (Hap0) or Tibet-0 (Hap1) genomic DNA. g Transient dual-luciferase assay in N. benthamiana. Data are mean ± SD from independent biological replicates (n = 3). The letters ‘a’ and ‘b’ indicate statistically significant differences by one-way ANOVA Duncan’s test (p = 0.0074, 0.0030, 0.0107, and 0.0042 < 0.05). Source data are provided as a Source Data file.