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. 2023 Oct 6;13:313. doi: 10.1038/s41398-023-02590-4

Table 5.

Results and details of recent studies investigating human adolescent substance use.

Reference Substance(s) studied Age of onset Mean or median age at testing(s) Number of users (% male) Brain Region Results
Tomek et al. [34] Alcohol 12–18 18 1,209 (51) Earlier alcohol initiation predicted later use in females, no effect in males
Squeglia et al. [92] Alcohol 19.6 75 (60) Cortex, corpus callosum, pons

-↓ white matter growth

- ↑ gray matter reduction

Luciana et al. [93] Alcohol 16.4 19.2 30 Cortex

-↑ decreases in cortical thickness

-↓ white matter development

Meda et al. [94] Alcohol 20.5 139 (51) Frontal gyrus, parahippocampus, anterior cingulate -↓ GMV
Infante et al. [95] Alcohol 17.5, 18.5, 19.5, 20.5 166 (53) Frontal regions -↓ GMV
El Marroun et al. [96] Alcohol 13.8 16.6 404 (47.8) Frontal cortex, cingulate cortex

-↓ GMV

-↓ increase in WMV

Sun et al. [97] Alcohol 16.8, 17.7, 18.7 185 (50.8) Cortex -Age-dependent changes in cortical thinning
Zhao et al. [98] Alcohol 20 160 (53.8) Whole brain -↓ white matter integrity
Shen et al. [99] Alcohol 24.8 62 (44) Whole brain ↓ fractional anisotropy in anterior corpus callosum in males, no effect in females
Phillips et al. [100] Alcohol 18.8 17.3, 18.3, 19.3 181 (51) Hippocampus, amygdala

-↓ hippocampal volume

-↑ amygdala volume

Murray et al. [111] Cannabis 19 12 (50) Adolescents showed greater behavioral and cognitive impairment and decreased P300 amplitude, relative to adults following acute THC exposure
Mustonen et al. [112] Cannabis 15.5 31.5 375 (44) ↑ risk of psychosis
Penzel et al. [115] Cannabis 17.4 23.8 102 (67) Cerebellum ↑ GMV, ↑ positive psychosis symptoms
Subramaniam et al. [127] Cannabis 15.5 20.4 18 (67) ACC ↓ GABA levels in adolescent cannabis users
Schaefer et al. [144] Cannabis <18 24, 29 3,762 ACE associated with lower educational attainment
Mokrysz et al. [148] Cannabis 15 526 (47) ACE not related to IQ or educational performance
Castellanos-Ryan et al. [150] Cannabis 14–17 20 127 (100) ACE associated with decline in verbal IQ and executive function tasks, and lower high school graduation.
Albaugh et al. [151] Cannabis 19 369 (51) PFC Accelerated cortical thinning, greater attentional impulsivity
Skumlien et al. [165] Cannabis 17.1 76 (49) Lower levels of anhedonia in moderate cannabis users
Skumlien et al. [166] Cannabis 17.2 32 (50) Ventral striatum No change in reward anticipation related activity
Clark et al. [159] Cannabis 14.3 743 (48) Identification of methylation markers in problematic cannabis users.
Kinnunen et al. [178] Nicotine 51 (48) Nicotine e-cigarette use increases risk of daily cigarette use
Dierker et al. [179] Nicotine, cannabis 14.7 (cannabis) 18.3 (59) Nicotine use significantly predicted later cannabis use
Kristjánsson et al. [180] Nicotine, Alcohol 15.5 587 (49) Electronic cigarette users were significantly more likely to use alcohol
Treur et al. [210] Nicotine 15, 42 2,111 (28) Increase in attentional problems in smokers relative to non-smoking twin
Sylvestre et al. [222] Nicotine 13 15 839 (44) Higher likelihood of developing nicotine dependence in girls than boys
Duan et al. [223] Nicotine 13–18 189 (51) Higher prvelance of past 30-day smoking in e-cigarette users than non-users. Relationship stronger in boys than girls.
Dai et al. [223] Nicotine 11.5 116 (52) Cortex Impaired cognitive performance, reduced cortical area and volume
Chaarni et al. [214] Nicotine 14 211 Cortex, corpus callosum

-↓ GMV

-Altered corpus callosum connectivity

Yu et al. [215] Nicotine 15.1 20.8 36 (100) Thalamus ↓ volume
Luo et al. [234] Alcohol, Cannabis 16 363 (49) Whole brain ↓ GMV in alcohol-cannabis co-users than in single-substance users
Infante et al. [235] Alcohol, Cannabis 16.4 (alcohol), 16.8 (cannabis) 27.5 Impaired cognitive function following AAE and ACE, but no additive effect
Thayer et al. [236] Alcohol, Cannabis 14–18 201 (74) Whole brain ↓ GMV in alcohol users, no effect of past 30-day cannabis use
Courtney et al. [244] Nicotine, Cannabis 16–22 111 (65.5) Cingulum tract, fornix tract ↓ WMV following nicotine and cannabis poly use

Note. WMV white matter volume, empty spaces indicate information not relevant to the study or not specified.