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. 2023 Oct 6;14(10):652. doi: 10.1038/s41419-023-06171-7

Fig. 1. PI3K/mTOR inhibitors overcome the primary radioresistance of SBC2 cells.

Fig. 1

A The time (12 h before IR) and concentration (50 nM) of PI3K/mTOR inhibitors could effectively inhibit PI3K/AKT/mTOR signaling. B The cell proliferation of cells treated with BEZ235 and GSK2126458 combined with or without IR were determined by CCK8 assay at 72 h after 6 Gy of X-ray irradiation. C Apoptotic cells were revealed by Hoechst staining. D, E The cell apoptosis of cells treated with PI3K/mTOR inhibitors combined with or without IR were detected by Annexin V/PI staining. F The survival fraction of SBC2 cells treated with IR combined with or without PI3K/mTOR inhibitors were determined by colony formation assay, and the number and size of SBC2 cell clones were detected under 2 Gy radiation exposure. G The expression of PARP and Cleaved-PARP across various groups were detected 24 h and 48 h after radiotherapy by immunoblotting.