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. 2023 Oct 6;14(10):652. doi: 10.1038/s41419-023-06171-7

Fig. 3. The difference between acquired radioresistant SCLC cells and its parental cell line H446.

Fig. 3

A The process of establishing SCLC cells with the acquired radioresistance was clearly shown. B The successful establishment of SCLC cells with the acquired radioresistance was validated. Under the exposure of 4 Gy, the expression of Cleaved-PARP and PARP were determined by immunoblotting and cell proliferation were determined by CCK8 assay. C The expression of key proteins related to PI3K/AKT/mTOR pathway between H446RR and H446 were determined by immunoblotting. D, E The protein and mRNA level of G6PD and GLUT1 between H446RR and H446 cells were determined by immunoblotting and qPCR. F G6PD activity between H446RR and H446 were determined. G Glucose intake and lactic acid production between H446RR and H446 were determined. H, I Differential expression analysis was carried out in the RNA-seq dataset between H446RR and H446 cells, and KEGG pathway analysis of these DEGs was performed. J Differential expression analysis was carried out in the RNA-seq dataset between acquired radioresistant murine SCLC cells with tdTomato and their parental cells, and KEGG pathway analysis of these DEGs was performed. K Differential expression analysis was carried out in CCLE datasets between primary radioresistant and radiosensitive SCLC cells, and KEGG pathway analysis of these DEGs was performed.