Fig. 4. PI3K/mTOR inhibitors increased the anti-tumor effect of IR in H446RR cells.

A The cell proliferation of cells across various groups were determined by CCK8 assay at 72 h after 6 Gy of X-ray irradiation. B The expression of PARP and Cleaved-PARP across various groups were determined by immunoblotting. C, D The apoptosis of H446RR cells in different groups were detected by Annexin V/PI staining. E, F The growth of H446RR cells in different groups were detected using Giemsa stain, although H446RR cells typically failed to form colonies when a single cell existed. G, H The migration of H446RR cells across various treatments were determined at 8 h, 12 h and 24 h by wound healing assay.