Skip to main content
. 2023 Oct 6;14:6242. doi: 10.1038/s41467-023-41770-0

Fig. 3. CaSR alters leukaemia progression and regulates self-renewal of AML LSC.

Fig. 3

a, b Kaplan–Meier style survival curves of mice transplanted with MLL-AF9+ (a; n = 9 (wildtype), n = 10 (CaSR KO), Log-rank test)) or BCR-ABL1+ (b; (n = 5 (wildtype), n = 7 (CaSR KO), Log-rank test)) wildtype (black) or CaSR KO (blue) BM. c, d Kaplan–Meier style survival curves of BALB/c mice transplanted with wildtype MLL-AF9+ (c) or BCR-ABL1 + -BM (d), co-transduced with empty vector- (black) or CaSR-overexpressing retrovirus (red) (n = 6 (empty vector), n = 8 (CaSR OE), Log-rank test). e Kaplan–Meier style survival curve of wildtype mice transplanted with wildtype or CaSR KO MLL-AF9+ BM. The wildtype donor BM was empty vector+ (black), and the CaSR KO BM was empty vector+ (blue) or overexpressed (OE) CaSR (red) (n = 16 (wildtype+empty vector), n = 16 (CaSR KO+empty vector), n = 8 (CaSR KO+CaSR OE), Log-rank test). f Kaplan-Meier-style survival curve of wildtype secondary mice transplanted with sorted MLL-AF9+ (GFP+) Lin− BM cells from primary recipients of MLL-AF9-transduced wildtype or CaSR KO BM (n = 10 (wildtype), n = 11 (CaSR KO), Log-rank test). From a–f n refers to individual mice. g Limiting dilution transplantation analysis for secondary wildtype recipient mice transplanted with sorted wildtype or CaSR KO AML cells (n = 4–5 mice per recipient group, Poisson statistics). h, i Colonies from the BM (h) or spleen (i) of wildtype mice transplanted with wildtype (black) or CaSR KO (blue) MLL-AF9-transduced BM (n = 4, two-tailed t test, mean ± SD). j Colonies from the serial replating of BM-derived cells from wildtype mice transplanted with MLL-AF9-transduced wildtype (black) or CaSR KO (blue) BM (n = 3, two-way ANOVA, Sidak’s test, mean ± SD). k Relative expression of stemness- and self-renewal-related genes in unsorted BM cells from mice transplanted with MLL-AF9-transduced wildtype, (black) CaSR KO (blue) and CaSR OE (red) leukaemia-initiating cells (n = 3 (CaSR KO, CaSR OE), n = 6 (wildtype), two-way ANOVA, Sidak’s test, mean ± SD). From h–k n refers to biological replicates. l Volcano plot summarising all differentially expressed genes in sorted MLL-AF9+ (GFP+) Lin− cells in the BM of wildtype recipient mice transplanted with MLL-AF9-transduced wildtype BM co-transduced with empty vector- or CaSR OE-expressing retrovirus. Upregulated versus downregulated genes are represented in red and blue, respectively. Data are presented as mean values ± SD. Source data are provided as a Source Data file. The schematics in a–g and j were created with BioRender.com.