Table 3.
Summary of secondary structure modifications observed using far-UV CD under thermal and chemical stress conditions. (n = 3).
| Stressor condition |
Avt |
Bio-1 |
Bio-2 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Thermal Stress (Temp; 50 ± 2 °C) | ||||||||||||
| Estimated secondary structure (%) | Ctrl | 1 d | 7 d | 14 d | Ctrl | 1 d | 7 d | 14 d | Ctrl | 1 d | 7 d | 14 d |
| Helix | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 |
| Antiparallel | 44.1 | 38.9 | 37.9 | 33.7 | 44.9 | 39.5 | 38.9 | 32.7 | 46.5 | 41.7 | 36 | 31.5 |
| Parallel | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Turn | 18.6 | 19.8 | 19.5 | 26.4 | 19.8 | 21.5 | 24.3 | 24.8 | 23.7 | 19.3 | 22.4 | 23.8 |
| Others |
37.3 |
41.3 |
42.5 |
37.9 |
35.3 |
39 |
36.8 |
41.9 |
29.8 |
39 |
41.7 |
44.7 |
| Stressor condition | Chemical Stress (pH; 3, 7, and 10 for 6 h) | |||||||||||
| Estimated secondary structure (%) | Ctrl | Acidic | Neutral | Basic | Ctrl | Acidic | Neutral | Basic | Ctrl | Acidic | Neutral | Basic |
| Helix | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Antiparallel | 44.2 | 43.2 | 44.1 | 38.6 | 44.9 | 43.3 | 44.8 | 36.6 | 46.5 | 45.4 | 46.4 | 35.5 |
| Parallel | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Turn | 18.5 | 16.1 | 18.6 | 22.3 | 19.8 | 16.3 | 19.9 | 23.2 | 23.7 | 25.4 | 23.8 | 24.3 |
| Others | 37.3 | 40.7 | 37.3 | 37.5 | 35.3 | 40.4 | 35.3 | 40.2 | 29.8 | 29.2 | 29.8 | 40.2 |