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. 2023 Aug 13;10(28):2301570. doi: 10.1002/advs.202301570

Figure 3.

Figure 3

Binding affinity of HAB‐30Kc19α‐RUNX2 to osteoblast mineral deposits and bone tissue section. A) Confirmation of fluorescent dye (OG488) conjugation to recombinant proteins by measuring the fluorescence intensity of unlabel1ed and label1ed recombinant proteins. B) Comparison of binding affinities of 30Kc19α‐RUNX2 and HAB‐30Kc19α‐RUNX2 to osteoblast mineral deposits. hMSCs were cultured with MSCGM or OM for 3 weeks. Then, cells were fixed with 4% PFA and treated with 1 µm of OG488‐label1ed recombinant proteins. Scale bar: 500 µm. C) Comparison of binding affinities of recombinant proteins to a bone tissue section. Mouse tissue sections were treated with 1 µm of OG488‐label1ed recombinant proteins. Bright‐field and green, fluorescent images were merged using ImageJ software. Scale bar: 500 µm.