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. 2023 Aug 6;10(28):2302130. doi: 10.1002/advs.202302130

Figure 6.

Figure 6

IRHOM2 is required for ZDHHC3 function over the course of NASH pathogenesis. a‐d) Records for body weight, liver weight, and liver weight/body weight ratio (%) (a), fasting blood glucose levels, fasting insulin levels (b), HOMA‐IR index (c), and glucose tolerance test (GTT) analysis (d) of the Zdhhc3‐Flox mice, Zdhhc3‐HepKO mice, Irhom2‐HepKO mice, and Irhom2/Zdhhc3‐HepDKO mice after 16‐weeks HFHC diet challenge (n = 10 mice per group; P < 0.05 versus Zdhhc3‐Flox HFHC group). e) Liver lipid contents including TG, TC, and NEFA in the indicated group (n = 10 mice per group; P < 0.05 versus Zdhhc3‐Flox HFHC group). f,g) Representative pictures of H&E staining (f), Oil red O staining (g), and corresponding NAS score in an indicated group (magnification, 100×; n = 10 images per group for each staining). h) Representative pictures of immunofluorescence analysis of Tgfβ & Collagen IV, and αSma & F4/80 coexpression, respectively (n = 10 images per group; P < 0.05 versus Zdhhc3‐Flox HFHC group). Scale bars, 100 µm. i,j) Records for serum pro‐inflammatory cytokines IL‐6, TNF‐α, IL‐18, CCL2, and IL‐1β contents (i) and liver function indicators including serum ALT, AST, and AKP contents (j) in indicated groups (n = 10 mice per group; P < 0.05 versus Zdhhc3‐Flox HFHC group). Data are expressed as mean ± SEM. The relevant experiments presented in this part were performed independently at least three times. Significance is determined by one‐way analysis of variance (ANOVA) followed by Dunnett's multiple comparisons test analysis. The P‐value < 0.05 was considered as significant difference.