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. 2023 Aug 16;10(28):2301641. doi: 10.1002/advs.202301641

Figure 1.

Figure 1

USP25 deficiency aggravates cerebral damage and neurological deficits in MCAO mice. A,B) Representative western blot images A) and quantification B) of USP25 protein abundance in the ischemic brain hemisphere on day 3 after MCAO. n = 5 mice per group. C) Relative transcription levels of USP25 in the ischemic brain hemisphere on day 2 after MCAO was determined by qRT‐PCR. n = 3 to 5 mice per group. D) Western blot analysis of USP25 expression in the brain of WT and USP25−/− mice. E,F) Representative TTC staining E) and percentages of infarct volume F) on day 0, 3, 7, 14 after MCAO. n = 3 to 4 mice per group. G–I) The protein levels of NeuN and PSD95 in the ischemic brain hemisphere on day 2 after MCAO were analyzed by western blot G). The relative protein levels of H) NeuN and I) PSD95 were calculated after normalization to GAPDH. n = 4 mice per group. J–O) Neurobehavioral functions were assessed with J) mNSS test, K) corner turning test, L,M) adhesive removal test, N) handgrip test, and O) rotarod test on day 1, 3, 7, 14 after MCAO. n = 4 to 10 mice per group. Data are displayed as the mean ± SEM.* P < 0.05; **P < 0.01; ***P < 0.001.