Figure 2.

Altered Treg fate and activation occur in the absence of BATF function in Tregs. A) Schematic representation of the generation of Treg‐specific BATF KO lineage tracer, TBKLT (Foxp3^Cre Batf^F/F mT/mG) mice. B–D) UMAP plot showing clusters identified by variable gene expression from sorted GFP⁺ Tregs from B) spleen, C) mesenteric LN [mLN], and D) colon of TBKLT mice (Foxp3^Cre Batf ^F/F) and WLT (Foxp3^Cre Batf^+/+ mT/mG) mice at 7 weeks of age. E–G) Violin plots showing expression of Treg‐specific genes in E) spleen, F) mLN, and G) colon in WLT control (green) and TBKLT (red) mice. H) Heatmap showing Z scores for the average expression of Treg‐specific genes in each cluster between WLT (green) and TBKLT mice (red).