Figure 3.

BATF is required for Treg accumulation and differentiation. A) Frequency of Foxp3⁺ Tregs in thymus, spleen, inguinal lymph nodes [iLN], lung, liver, and colon in TBKLT mice compared to control mice. Foxp3 expression is conferred by direct staining with and detection of intranuclear fluorophore‐conjugated antibody. B) Similar to A, but showing average expression of FOXP3 in CD4. C) Frequency of aTregs (CD62^low CD44^high) and rTregs (CD62L^highCD44^low) in spleen and iLN of TBKLT mice and control mice. D) Frequency of KLRG1⁺ CD44⁺ FOXP3⁺ Tregs in spleen, iLN, and colon in TBKLT mice compared to WLT control mice. E) Average expression of ICOS in CD44⁺ FOXP3⁺ Tregs in TBKLT mice and control mice. All data are from mice between 5 to 11 weeks of age and are pooled from at least three independent experiments with 2–3 mice per group. Data here are shown ± SEM. *p < 0.05, ^** p < 0.01, and ^*** p < 0.001 (two tailed unpaired t‐test).