Figure 4.

BATF is intrinsically required for Treg homeostasis and competitive fitness. A) Schematic representation of TBKLT mosaic (Foxp3^Cre/WT Batf^F/F) mice compared to control (Foxp3^Cre/Cre Batf^+/+) mice. B) Frequency of activated CD4 T cells in TBKLT mosaic mice compared to control mice. C) Frequency of Foxp3⁺ GFP⁻ (BATF sufficient) and Foxp3⁺ GFP⁺ (BATF deficient) Tregs in spleen, iLN, liver, and colons of female TBKLT mosaic mice. FOXP⁺ expression is conferred by direct staining with and detection of intranuclear fluorophore‐conjugated antibody. Data are from pooled two independent experiments using female TBKLT mosaic and control mice, around 3–4 months of age, and 2 mice per group. Data here are shown ± SEM. *p < 0.05, ^** p < 0.01, and ^*** p < 0.001 (two tailed unpaired t‐test).