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. 2023 Aug 10;10(28):2303496. doi: 10.1002/advs.202303496

Figure 1.

Figure 1

Domain insertion profiling of functionally and structurally diverse proteins. A) Flow chart of the domain insertion screening workflow. B) Overview of the screened PDZ‐domain insertion libraries. The depicted structures of the parent proteins are AF2 predictions. PDB‐ID of PDZ: 1Z86. C) Enrichment score histograms for the different candidate proteins are shown. The Log2 norm. read counts correspond to the fraction of reads after enrichment normalized to the fraction of read counts within the initial library. Data from the four candidate proteins AraC, Flp, TVMV protease, and SigF with PDZ domain inserts are shown. Enrichments are mapped to the respective insertion site as indicated by the position of the acceptor proteins preceding the insertion. Light green, dark green: individual replicates. Grey: variants with zero reads after enrichment. Red: variants missing in the initial library. Insertion sites correspond to residues preceding the inserted domain.