Antibodies |
|
Anti-human CD45 (Clone H130) |
Biolegend |
CAT#304026, RFID: AB_893337
|
Anti-human TCR αβ (Clone I26) |
Biolegend |
CAT#306716, RRID: AB_1953257
|
Anti-human TCR Vβ13.1 (Clone H131) |
Biolegend |
CAT#362403, RRID: AB_2564031
|
Anti-human CD4 (Clone OKT4) |
Biolegend |
CAT#317414, RRID: AB_571959
|
Anti-human CD8 (Clone SK1) |
Biolegend |
CAT#344714, RRID: AB_2044006
|
Anti-human CD19 (Clone SJ25C1) |
Biolegend |
CAT#363005, RRID: AB_2564127
|
Anti-human CD31 (Clone WM59) |
Biolegend |
CAT#303117, RRID: AB_2114314
|
Anti-human CD34 (Clone 581) |
Biolegend |
CAT#555822, RRID: AB_396151
|
Anti-human CD44 (Clone BJ18) |
Biolegend |
CAT#397503, RRID: AB_2814372
|
Anti-human CD24 (Clone ML5) |
Biolegend |
CAT#311113, RRID: AB_2561283
|
Anti-human CD133 (Clone clone7) |
Biolegend |
CAT#372805, RRID: AB_2632881
|
Anti-human CD117 (Clone 104D2) |
Biolegend |
CAT#313227, RRID: AB_2566214
|
Anti-human CD112 (Clone TX31) |
Biolegend |
CAT#337409, RRID: AB_2174163
|
Anti-human CD155 (Clone SKII.4) |
Biolegend |
CAT#337613, RRID: AB_2565746
|
Anti-human CD14 (Clone HCD14) |
Biolegend |
CAT#325608, RRID: AB_830681
|
Anti-human CD1d (Clone 51.1) |
Biolegend |
CAT#350308, RRID: AB_10642829
|
Anti-human CD11b (Clone ICRF44) |
Biolegend |
CAT#301330, RRID: AB_2561703
|
Anti-human CD56 (Clone HCD56) |
Biolegend |
CAT#362545, RRID: AB_2565963
|
Anti-human CD206 (Clone 15-2) |
Biolegend |
CAT#321110, RRID: AB_571885
|
Anti-human CD163 (Clone GHI/61) |
Biolegend |
CAT#333621, RRID: AB_2563611
|
Anti-human CD86 (Clone IT2.2) |
Biolegend |
CAT#374209, RRID: AB_2728391
|
Anti-human NKp30 (Clone P30-15) |
Biolegend |
CAT#325207, RRID: AB_756111
|
Anti-human NKp44 (Clone P44-8) |
Biolegend |
CAT#325107, RRID: AB_756099
|
Anti-human NKG2D (Clone 1D11) |
Biolegend |
CAT#320812, RRID: AB_2234394
|
Anti-human DNAM-1 (Clone 11A8) |
Biolegend |
CAT#338312, RRID: AB_2561952
|
Anti-human Granzyme B (Clone QA16A02) |
Biolegend |
CAT#372204, RRID: AB_2687028
|
Anti-human Perforin (Clone dG9) |
Biolegend |
CAT#308126, RRID: AB_2572049
|
Anti-human b2-microglobulin (B2M) (Clone 2M2) |
Biolegend |
CAT#316312, RRID: AB_10641281
|
Anti-human HLA-DR (Clone L243) |
Biolegend |
CAT#307618, RRID: AB_493586
|
Anti-human HLA-A,B,C (Clone W6/32) |
Biolegend |
CAT#311421, RRID: AB_1501265
|
Anti-human MICA/MICB (Clone 6D4) |
Biolegend |
CAT#320908, RRID: AB_493195
|
Anti-human CTAG1B (W19067B) |
Biolegend |
CAT#382302, RRID: AB_2922615
|
Anti-human CA125 (Clone 618F) |
Biolegend |
CAT#666904, RRID: AB_2629540
|
Anti-SOX2 (Clone 14A6A34) |
Biolegend |
CAT#656112, RRID: AB_2566189
|
Anti-Oct3/4 (Clone 3A2A20) |
Biolegend |
CAT#653704, RRID: AB_2562018
|
LEAF purified anti-human NKG2D antibody (Clone 1D11) |
Biolegend |
CAT#320810, RRID: AB_2133276
|
LEAF purified anti-human CD1d antibody (Clone 51.1) |
Biolegend |
CAT#350304, RRID: AB_10641291
|
Mouse IgG1, k isotype control antibody (Clone MOPC-21) |
Biolegend |
CAT#400124, RRID: AB_2890215
|
LEAF purified Mouse IgG2b, k isotype ctrl (Clone MG2b-57) |
Biolegend |
CAT#401201, RRID: AB_2744505
|
Human Fc Receptor Blocking Solution (TrueStain FcX) |
Biolegend |
CAT#422302, RRID: AB_2818986
|
Goat anti-mouse IgG (minimal x-reactivity) Antibody |
Biolegend |
CAT#405305, RRID: AB_315008
|
Goat anti-rat IgG (minimal x-reactivity) Antibody |
Biolegend |
CAT#405413, RRID: AB_10661733
|
Donkey anti-rabbit IgG (minimal x-reactivity) Antibody |
Biolegend |
CAT#406421, RRID: AB_2563484
|
Streptavidin |
Biolegend |
CAT#405207 |
Anti-human TCR Va24-Jb18 (Clone 6B11) |
BD Biosciences |
CAT#552825, RRID: AB_394478
|
LEAF purified anti-human DNAM-1 antibody (Clone DX11) |
BD Biosciences |
CAT#559786, RRID: AB_397327
|
Mouse Fc Block (anti-mouse CD16/32) |
BD Biosciences |
CAT#553142, RRID: AB_394657
|
Anti-human mesothelin (MSLN) (Clone 420411) |
R&D Systems |
CAT#FAB32652P, RRID: AB_1151946
|
Anti-human fibroblast activation protein alpha/FAP (Clone 427819) |
R&D Systems |
CAT# MAB3715-SP |
Anti-human ULBP-1 (Clone 170818) |
R&D Systems |
CAT#FAB1380P, RRID: AB_2687471
|
Anti-human ULBP-2,5,6 (Clone 165903) |
R&D Systems |
CAT#FAB1298A, RRID: AB_2257142
|
Goat anti-Mouse IgG F(ab')2 Secondary Antibody, Biotin |
ThermoFisher |
CAT#31803, RRID: AB_228311
|
Anti-Nanog (Clone EPR2027-2) |
Abcam |
CAT#ab109250, RRID: AB_10863442
|
Anti-human Vβ11 |
Beckman-Coulter |
CAT#A66905 |
|
Bacterial and virus strains |
|
Lenti/iNKT-sr39TK |
This paper |
N/A |
Lenti/MCAR |
This paper |
N/A |
Lenti/1G4-TK |
This paper |
N/A |
|
Biological samples |
|
Human peripheral blood mononuclear cells (PBMCs) |
UCLA |
N/A |
Human cord blood CD34+ hematopoietic stem and progenitor cells (HSCs) |
UCLA |
N/A |
Human ovarian cancer patient samples |
UCLA |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
Streptavidin-HRP conjugate |
Invitrogen |
CAT#SA10001 |
Recombinant human IL-2 |
Peprotech |
CAT#200–02 |
Recombinant human IL-3 |
Peprotech |
CAT#200–03 |
Recombinant human IL-7 |
Peprotech |
CAT#200–07 |
Recombinant human IL-15 |
Peprotech |
CAT#200–15 |
Recombinant human Flt3-Ligand |
Peprotech |
CAT#300–19 |
Recombinant human SCF |
Peprotech |
CAT#300–07 |
Recombinant human TPO |
Peprotech |
CAT#300–18 |
Recombinant human GM-CSF |
Peprotech |
CAT#300–03 |
L-ascorbic acid 2-phosphate |
Sigma |
CAT#A8960-5G |
B27" Supplement (50X), serumfree |
ThermoFisher |
CAT#17504044 |
a-Galactosylceramide (KRN7000) |
Avanti Polar Lipids |
SKU#867000P-1mg |
X-VIVO 15 Serum-free Hematopoietic Cell Medium |
Lonza |
CAT#04–418Q |
UltraCULTURE media |
Lonza |
CAT#BP12725F |
RPMI1640 cell culture medium |
Corning Cellgro |
CAT#10-040-CV |
DMEM cell culture medium |
Corning Cellgro |
CAT#10-013-CV |
Fetal Bovine Serum (FBS) |
Sigma |
CAT#F2442 |
MACS BSA stock solution |
Miltenyi |
CAT#130-091-376 |
30% BSA |
Gemini |
CAT#50-753-3079 |
Penicillin-Streptomycine-Glutamine (P/S/G) |
Gibco |
CAT#10378016 |
Penicillin: streptomycin (pen:strep) solution (P/S) |
Gemini Bio-products |
CAT#400–109 |
MEM non-essential amino acids (NEAA) |
Gibco |
CAT#11140050 |
HEPES Buffer Solution |
Gibco |
CAT#15630056 |
Sodium Pyruvate |
Gibco |
CAT#11360070 |
Beta-Mercaptoethanol |
Sigma |
SKU#M6250 |
Normocin |
InvivoGen |
CAT#ant-nr-2 |
Cell Fixation/Permeabilization Kit |
BD Biosciences |
CAT#554714 |
RetroNectin recombination human fibronectin fragment, 2.5mg |
Takara |
CAT#T100B |
Phosphate Buffered Saline (PBS) pH 7.4 (1X) |
Gibco |
CAT#10010–023 |
Poloxamer Synperonic F108 |
Sigma |
CAT#07579–250G-F |
Prostaglandin E2 |
Cayman Chemical |
CAT#14-190-1 |
Collagenase-Type I |
ThermoFisher |
CAT#17100017 |
Dispase II |
ThermoFisher |
CAT#17105041 |
DNase I |
Sigma-Aldrich |
CAT #10104159001 |
Fixable Viability Dye eFluor506 affymetrix |
eBioscience |
CAT#65-0866-14 |
|
Critical commercial assays |
|
Human CD34 MicroBeads Kit |
Miltenyi Biotec |
CAT#130-046-703 |
Human CD14 MicroBeads Kit |
Miltenyi Biotec |
CAT#130-050-201 |
Human Anti-iNKT MicroBeads |
Miltenyi Biotec |
CAT#130-094-842 |
Human tumor cell isolation kit |
Miltenyi Biotec |
CAT#130-108-339 |
Fixation/Permeabilization Solution Kit |
BD Sciences |
CAT#55474 |
Foxp3 / Transcription Factor Staining Buffer Set |
eBioscience |
CAT#00-5523-00 |
StemSpan™™ Lymphoid Differentiation Coating Material (100X) |
Stem Cell Technologies |
CAT#9925 |
StemSpan™ SFEM II |
Stem Cell Technologies |
CAT#9605 |
ImmunoCult" Human CD3/CD28/CD2 T Cell Activator |
Stem Cell Technologies |
CAT#10970 |
TransIT-Lenti Transfection Reagent |
Mirus Bio |
CAT#MIR 6600 |
Amicon Ultra-15 Centrifugal Filter Unit |
Millipore Sigma |
CAT#UFC910024 |
Cryostor cell cryopreservation media |
Sigma |
CAT#C2874-100ML |
|
Recombinant DNA |
|
Vector: parental lentivector pMNDW |
97,98
|
N/A |
|
Software and algorithms |
|
FlowJo Software 9 |
FlowJo |
https://www.flowjo.com/solutions/flowjo/downloads |
Prism 8 |
Graphpad |
https://www.graphpad.com/scientific-software/prism/ |