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. 2023 Sep 19;26(10):107952. doi: 10.1016/j.isci.2023.107952
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-human CD45 (Clone H130) Biolegend CAT#304026, RFID: AB_893337
Anti-human TCR αβ (Clone I26) Biolegend CAT#306716, RRID: AB_1953257
Anti-human TCR Vβ13.1 (Clone H131) Biolegend CAT#362403, RRID: AB_2564031
Anti-human CD4 (Clone OKT4) Biolegend CAT#317414, RRID: AB_571959
Anti-human CD8 (Clone SK1) Biolegend CAT#344714, RRID: AB_2044006
Anti-human CD19 (Clone SJ25C1) Biolegend CAT#363005, RRID: AB_2564127
Anti-human CD31 (Clone WM59) Biolegend CAT#303117, RRID: AB_2114314
Anti-human CD34 (Clone 581) Biolegend CAT#555822, RRID: AB_396151
Anti-human CD44 (Clone BJ18) Biolegend CAT#397503, RRID: AB_2814372
Anti-human CD24 (Clone ML5) Biolegend CAT#311113, RRID: AB_2561283
Anti-human CD133 (Clone clone7) Biolegend CAT#372805, RRID: AB_2632881
Anti-human CD117 (Clone 104D2) Biolegend CAT#313227, RRID: AB_2566214
Anti-human CD112 (Clone TX31) Biolegend CAT#337409, RRID: AB_2174163
Anti-human CD155 (Clone SKII.4) Biolegend CAT#337613, RRID: AB_2565746
Anti-human CD14 (Clone HCD14) Biolegend CAT#325608, RRID: AB_830681
Anti-human CD1d (Clone 51.1) Biolegend CAT#350308, RRID: AB_10642829
Anti-human CD11b (Clone ICRF44) Biolegend CAT#301330, RRID: AB_2561703
Anti-human CD56 (Clone HCD56) Biolegend CAT#362545, RRID: AB_2565963
Anti-human CD206 (Clone 15-2) Biolegend CAT#321110, RRID: AB_571885
Anti-human CD163 (Clone GHI/61) Biolegend CAT#333621, RRID: AB_2563611
Anti-human CD86 (Clone IT2.2) Biolegend CAT#374209, RRID: AB_2728391
Anti-human NKp30 (Clone P30-15) Biolegend CAT#325207, RRID: AB_756111
Anti-human NKp44 (Clone P44-8) Biolegend CAT#325107, RRID: AB_756099
Anti-human NKG2D (Clone 1D11) Biolegend CAT#320812, RRID: AB_2234394
Anti-human DNAM-1 (Clone 11A8) Biolegend CAT#338312, RRID: AB_2561952
Anti-human Granzyme B (Clone QA16A02) Biolegend CAT#372204, RRID: AB_2687028
Anti-human Perforin (Clone dG9) Biolegend CAT#308126, RRID: AB_2572049
Anti-human b2-microglobulin (B2M) (Clone 2M2) Biolegend CAT#316312, RRID: AB_10641281
Anti-human HLA-DR (Clone L243) Biolegend CAT#307618, RRID: AB_493586
Anti-human HLA-A,B,C (Clone W6/32) Biolegend CAT#311421, RRID: AB_1501265
Anti-human MICA/MICB (Clone 6D4) Biolegend CAT#320908, RRID: AB_493195
Anti-human CTAG1B (W19067B) Biolegend CAT#382302, RRID: AB_2922615
Anti-human CA125 (Clone 618F) Biolegend CAT#666904, RRID: AB_2629540
Anti-SOX2 (Clone 14A6A34) Biolegend CAT#656112, RRID: AB_2566189
Anti-Oct3/4 (Clone 3A2A20) Biolegend CAT#653704, RRID: AB_2562018
LEAF purified anti-human NKG2D antibody (Clone 1D11) Biolegend CAT#320810, RRID: AB_2133276
LEAF purified anti-human CD1d antibody (Clone 51.1) Biolegend CAT#350304, RRID: AB_10641291
Mouse IgG1, k isotype control antibody (Clone MOPC-21) Biolegend CAT#400124, RRID: AB_2890215
LEAF purified Mouse IgG2b, k isotype ctrl (Clone MG2b-57) Biolegend CAT#401201, RRID: AB_2744505
Human Fc Receptor Blocking Solution (TrueStain FcX) Biolegend CAT#422302, RRID: AB_2818986
Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend CAT#405305, RRID: AB_315008
Goat anti-rat IgG (minimal x-reactivity) Antibody Biolegend CAT#405413, RRID: AB_10661733
Donkey anti-rabbit IgG (minimal x-reactivity) Antibody Biolegend CAT#406421, RRID: AB_2563484
Streptavidin Biolegend CAT#405207
Anti-human TCR Va24-Jb18 (Clone 6B11) BD Biosciences CAT#552825, RRID: AB_394478
LEAF purified anti-human DNAM-1 antibody (Clone DX11) BD Biosciences CAT#559786, RRID: AB_397327
Mouse Fc Block (anti-mouse CD16/32) BD Biosciences CAT#553142, RRID: AB_394657
Anti-human mesothelin (MSLN) (Clone 420411) R&D Systems CAT#FAB32652P, RRID: AB_1151946
Anti-human fibroblast activation protein alpha/FAP (Clone 427819) R&D Systems CAT# MAB3715-SP
Anti-human ULBP-1 (Clone 170818) R&D Systems CAT#FAB1380P, RRID: AB_2687471
Anti-human ULBP-2,5,6 (Clone 165903) R&D Systems CAT#FAB1298A, RRID: AB_2257142
Goat anti-Mouse IgG F(ab')2 Secondary Antibody, Biotin ThermoFisher CAT#31803, RRID: AB_228311
Anti-Nanog (Clone EPR2027-2) Abcam CAT#ab109250, RRID: AB_10863442
Anti-human Vβ11 Beckman-Coulter CAT#A66905

Bacterial and virus strains

Lenti/iNKT-sr39TK This paper N/A
Lenti/MCAR This paper N/A
Lenti/1G4-TK This paper N/A

Biological samples

Human peripheral blood mononuclear cells (PBMCs) UCLA N/A
Human cord blood CD34+ hematopoietic stem and progenitor cells (HSCs) UCLA N/A
Human ovarian cancer patient samples UCLA N/A

Chemicals, peptides, and recombinant proteins

Streptavidin-HRP conjugate Invitrogen CAT#SA10001
Recombinant human IL-2 Peprotech CAT#200–02
Recombinant human IL-3 Peprotech CAT#200–03
Recombinant human IL-7 Peprotech CAT#200–07
Recombinant human IL-15 Peprotech CAT#200–15
Recombinant human Flt3-Ligand Peprotech CAT#300–19
Recombinant human SCF Peprotech CAT#300–07
Recombinant human TPO Peprotech CAT#300–18
Recombinant human GM-CSF Peprotech CAT#300–03
L-ascorbic acid 2-phosphate Sigma CAT#A8960-5G
B27" Supplement (50X), serumfree ThermoFisher CAT#17504044
a-Galactosylceramide (KRN7000) Avanti Polar Lipids SKU#867000P-1mg
X-VIVO 15 Serum-free Hematopoietic Cell Medium Lonza CAT#04–418Q
UltraCULTURE media Lonza CAT#BP12725F
RPMI1640 cell culture medium Corning Cellgro CAT#10-040-CV
DMEM cell culture medium Corning Cellgro CAT#10-013-CV
Fetal Bovine Serum (FBS) Sigma CAT#F2442
MACS BSA stock solution Miltenyi CAT#130-091-376
30% BSA Gemini CAT#50-753-3079
Penicillin-Streptomycine-Glutamine (P/S/G) Gibco CAT#10378016
Penicillin: streptomycin (pen:strep) solution (P/S) Gemini Bio-products CAT#400–109
MEM non-essential amino acids (NEAA) Gibco CAT#11140050
HEPES Buffer Solution Gibco CAT#15630056
Sodium Pyruvate Gibco CAT#11360070
Beta-Mercaptoethanol Sigma SKU#M6250
Normocin InvivoGen CAT#ant-nr-2
Cell Fixation/Permeabilization Kit BD Biosciences CAT#554714
RetroNectin recombination human fibronectin fragment, 2.5mg Takara CAT#T100B
Phosphate Buffered Saline (PBS) pH 7.4 (1X) Gibco CAT#10010–023
Poloxamer Synperonic F108 Sigma CAT#07579–250G-F
Prostaglandin E2 Cayman Chemical CAT#14-190-1
Collagenase-Type I ThermoFisher CAT#17100017
Dispase II ThermoFisher CAT#17105041
DNase I Sigma-Aldrich CAT #10104159001
Fixable Viability Dye eFluor506 affymetrix eBioscience CAT#65-0866-14

Critical commercial assays

Human CD34 MicroBeads Kit Miltenyi Biotec CAT#130-046-703
Human CD14 MicroBeads Kit Miltenyi Biotec CAT#130-050-201
Human Anti-iNKT MicroBeads Miltenyi Biotec CAT#130-094-842
Human tumor cell isolation kit Miltenyi Biotec CAT#130-108-339
Fixation/Permeabilization Solution Kit BD Sciences CAT#55474
Foxp3 / Transcription Factor Staining Buffer Set eBioscience CAT#00-5523-00
StemSpan™™ Lymphoid Differentiation Coating Material (100X) Stem Cell Technologies CAT#9925
StemSpan™ SFEM II Stem Cell Technologies CAT#9605
ImmunoCult" Human CD3/CD28/CD2 T Cell Activator Stem Cell Technologies CAT#10970
TransIT-Lenti Transfection Reagent Mirus Bio CAT#MIR 6600
Amicon Ultra-15 Centrifugal Filter Unit Millipore Sigma CAT#UFC910024
Cryostor cell cryopreservation media Sigma CAT#C2874-100ML

Recombinant DNA

Vector: parental lentivector pMNDW 97,98 N/A

Software and algorithms

FlowJo Software 9 FlowJo https://www.flowjo.com/solutions/flowjo/downloads
Prism 8 Graphpad https://www.graphpad.com/scientific-software/prism/