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. 2023 Aug 29;9(9):e19601. doi: 10.1016/j.heliyon.2023.e19601

Table 1.

Definitive endoderm differentiation medium.

DAY MEDIUM/SUPPLEMENTS (For 24- and 12-well plate use 0.5 and 1 mL medium/well, respectively, for differentiation)
1 RPMI 1640 + 0% FBS + activin A (100 ng/mL) + CHIR9902, (5 μM) + Y27632 (10 μM)
2 RPMI 1640 + 0.2% FBS + activin A (100 ng/mL)
3–4 RPMI 1640 + 2% FBS + activin A (100 ng/mL)