Key resources table
| REAGENT | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-FOXJ1 | eBioscience | 14-9965-82 |
| Mouse anti-surfactant protein B (SFTPB) | Seven Hills Bioreagents | Wmab-1B9 |
| Rabbit anti-prosurfactant protein C (proSP-C) | Millipore | AB3786 |
| Rabbit anti-RAGE | Abcam | ab216329 |
| Mouse anti-Hop (E−1) | Santa Cruz | Sc-398703 |
| Rabbit anti-NKX2.1 | Abcam | ab76013 |
| Rabbit anti-SOX9 | Millipore | AB5535 |
| Rabbit anti-ECAD | Cell Signaling Technology | 3195 |
| Mouse anti-CC10 (SCGB1A1) | Santa Cruz | sc-365992 |
| Rabbit Anti-ACE2 | Novus Biologicals | NBP2-67692 |
| Rabbit anti-TMPRSS2 | Abcam | ab92323 |
| Rabbit anti-Ki67 | Millipore | AB9260 |
| Mouse anti-GFAP | Sigma-Aldrich | G3893 |
| Rabbit anti-SOX2 | Abcam | ab97959 |
| Mouse anti-β-tubulin III | Abcam | ab7751-100 |
| Chicken anti-Nestin | Abcam | ab134017 |
| Chicken anti-MAP2 | Abcam | ab5392 |
| Alexa Fluor 488, 594, and 647 conjugated secondary antibodies | Molecular Probes/Invitrogen | A11032, A11034, A11012, A11039, A11001 |
| Antifade mounting medium with DAPI (Vectashield) | Vector Laboratories | H-1200 |
| Chemicals | ||
| 1-Thioglycerol | Sigma-Aldrich | M6145 |
| l-Ascorbic acid | Tocris Bioscience | 4055 |
| Bovine serum albumin (BSA) | Fisher Scientific | BP9703–100 |
| Protease and phosphatase inhibitor cocktail | Pierce | A32955 |
| Bio-Rad DC protein assay kit | Bio-Rad | 5000112 |
| Chemiluminescence substrate | Thermo Scientific | 34580 |
| Optimal cutting temperature (OCT) compound | Tissue-Tech | 4583 |
| RIPA buffer | Teknova | R3792 |
| Falcon™ Cell Culture Inserts | BD, Falcon | 08–770 |
| β-Mercaptoethanol | Sigma-Aldrich | M7522 |
| Immun-Blot PVDF membrane | Bio-Rad Laboratories | 1620177 |
| Growth Factors | ||
| Activin A | R&D Systems | 338-AC |
| FGF10 (recombinant human fibroblast growth factor 10) | R&D Systems | 345-FG |
| FGF4 (recombinant human fibroblast growth factor 4) | R&D Systems | 7460-F4 |
| FGF7 (recombinant human fibroblast growth factor 7) | R&D Systems | 251-KG/CF |
| NOGGIN | R&D Systems | 6057 |
| Smoothened agonist (SAG) | Enzo Life Sciences | ALX-270-426-M001 |
| Epidermal growth factor (EGF) | Sigma-Aldrich | E9644 |
| Basic fibroblast growth factor (bFGF) | Sigma-Aldrich | F0291 |
| Small molecules | ||
| SB431542 (selective inhibitor of the transforming growth factor-β (TGF-β) type I receptor) | TOCRIS | 1614 |
| CHIR9902 (GSK3β inhibitor/Wnt activator) | TOCRIS | 4423 |
| Y-27632 (ROCK inhibitor) | Sigma-Aldrich | SCM075 |
| Cell Lines | ||
| Human induced pluripotent stem cells (hiPSCs) | Cell Application | hiPS11-10 |
| 293FT (HEK) | American Type Culture Collection (ATCC) | CRL-1573 |
| Calu-3 human lung epithelial cell | ATCC | HTB-55 |
| Culture Media | ||
| Advanced DMEM | Thermo Fisher Scientific | 12491015 |
| CHIR99021 | Stem cell Technologies | 72054 |
| GlutaMAX (100X) | Thermo Fisher Scientific | 35050061 |
| HEPES (1 M) | Thermo Fisher Scientific | 15630080 |
| mTeSR | Stem cell Technologies | 85850 |
| N-2 supplement | Thermo Fisher Scientific | 17502048 |
| Penicillin-streptomycin (100X) | Thermo Fisher Scientific | 15140122 |
| RPMI 1640 medium | Thermo Fisher Scientific | 11875119 |
| Matrigel basement membrane matrix (growth factor reduced) | Corning | 354230 |
| Matrigel basement membrane matrix | Corning | 354234 |
| Versene solution | Thermo Fisher Scientific | 15040066 |
| DMEM/F12 | Thermo Fisher Scientific | 11320082 |
| MEM nonessential amino acids (MEM-NEAA) | Thermo Fisher Scientific | 11140050 |
| B-27 supplement without ATRA | Thermo Fisher Scientific | 12587010 |
| B-27 supplement with ATRA | Thermo Fisher Scientific | 17504044 |
| GlutaMAX, 200 mM | Thermo Fisher Scientific | 35050061 |
| l-Glutamine | Thermo Fisher Scientific | A2916801 |
| Dispase | Thermo Fisher Scientific | 17105-0412q |
| Collagenase IV | Thermo Fisher Scientific | 17104019 |
| Fetal bovine serum (FBS) | Thermo Fisher Scientific | 10438–018 |
| Bovine serum albumin (BSA) | Fisher Scientific | BP9703-100 |