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. 2023 Sep 18;44(5):848–859. doi: 10.24272/j.issn.2095-8137.2023.080

Figure 3.

Figure 3

Effects of ATF6 knockdown on ER function in high-temperature (HT)-embryos

A: Typical images of ER-tracker distribution at 4C stage after HT exposure and ATF6 dsRNA microinjection. Blue, DAPI; Green, ER-tracker. Bar: 20μm. B: Typical images of CHOP intensity at 4C stage after HT exposure and ATF6 dsRNA microinjection. Blue, DAPI; Red, CHOP. Bar: 20 μm. C: Abnormal ER distribution rate at 4C stage after HT exposure and ATF6 dsRNA microinjection. *: P<0.05. D: Fluorescence intensity of CHOP at 4C stage after HT exposure and ATF6 dsRNA microinjection. *: P<0.05; **: P<0.01. E: Typical images of Fluo-4-AM and Rhod-2-AM intensity at BL stage after HT exposure and ATF6 dsRNA microinjection. Red, Rhod-2-AM; Green, Fluo-4-AM. Bar: 100 μm. F: Fluorescence intensity of Fluo-4-AM at BL stage after HT exposure and ATF6 dsRNA microinjection. **: P<0.01. G: Fluorescence intensity of Rhod-2-AM at BL stage after HT exposure and ATF6 dsRNA microinjection. *: P<0.05.