Table 8.
Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-E.coli LPS antibody | Abcam | Cat# ab35654 |
| Anti E.coli OmpA Polyclonal Antibody | Antibody research | Cat# 111120 |
| Anti-mouse IgG, HRP-linked | Cell Signaling | Cat# 7076 |
| BV711 Anti-Human CD4 | BioLegend | Cat# 317439, RRID: AB_11219404 |
| PE-Cy7 Mouse anti-human CD279 (PD-1) | BD Biosciences | Cat# 561272, RRID: AB_10611585 |
| APC anti-human CD366 (Tim-3) | BioLegend | Cat# 345011, RRID: AB_2561717 |
| Chemicals, peptides, and recombinant proteins | ||
| ECL detection reagent | GE Healthcare | Cat# RPN2235 |
| DNase I | Nippon Gene | Cat# 314-08071 |
| KAPA HiFi HS ReadyMix | Kapa Biosystems | Cat# KK2602 |
| AMPure XP | Beckman Coulter | Cat# A63880 |
| Human TruStain FcX | BioLegend | Cat# 422302 |
| Critical commercial assays | ||
| Total Exosome Isolation Reagent (from serum) | Thermo Fisher | Cat# 4478360 |
| QIAamp® Circulating Nucleic Acid Kit | Qiagen | Cat# 55114 |
| Qubit ® dsDNA HS Assay Kits | Thermo Fisher | Cat# Q32851 |
| Bioanalyzer High Sensitivity DNA Kit | Agilent | Cat# 5067-4626 |
| Zombie NIR™ Fixable Viability Kit | Biolegend | Cat# 423105 |
| Software and algorithms | ||
| QIIME | Illumina | http://qiime.org/1.3.0/index.html# |
| Galaxy | The Huttenhower Lab | http://huttenhower.sph.harvard.edu/galaxy/ |
| Izon Control Suite | Izon | n/a |
| BD FACSDiva | BD Biosciences | n/a |
| JMP | SAS Institute | V16.1 |
| Prism | GraphPad | V9.2 |
| Other | ||
| qNano Gold | Izon | n/a |
| HT7800 | HITACHI | n/a |
| Amersham Imager 680 | GE Healthcare | n/a |
| LSRFortessa X20 flow cytometer | BD Biosciences | n/a |