FIGURE 4:

Apparent molecular weight of uWNK3 as a function of back-pressure. (A) Gravity gel filtration of uWNK3 on Sephadex^TM 75 resin in 150 mM NaCl and 25°C. Elution volumes of standard albumin (67 kDa, 16.0 mL) and ovalbumin (43 kDa, 17.0 mL; red vertical dashed lines). Gel filtration of uWNK3 as run on the Acta FPLC at 290 kPa Sephadex^TM 75 column. Albumin standard (63.4 mL); ovalbumin (69.6 mL). Gel Filtration of pWNK3 in the same buffer (standard, ovalbumin [43 kDa, 64.3 mL]). (B) SEC-MALS data collected with a backpressure of 250 kPA. (C) Analytical ultracentrifugation (9–19 MPa) at two uWNK3 concentrations, 2.1 mg/mL (52 mM, black) and 0.8 mg/mL (19.2 mM, red). The calculated apparent calculated molecular weight was 38.5 kg/mol.