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. 2023 Sep 21;34(11):ar109. doi: 10.1091/mbc.E23-03-0113

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FIGURE 6: — DSS crosslinks of wild-type uWNK3 formed without and with added hydrostatic pressure. Crosslinks unique to (A) unpressurized and (B) pressurized WNK3 visualized as a function of sequence in xVis (Grimm et al., 2015). Orange, intramolecular crosslinks, magenta, inter-subunit crosslinks. (C) Crosslinks in unpressurized uWNK3 mapped onto the crystallographic dimer of uWNK1 (PDB file 6CN9). Subunit A, green; B, cyan. The AL from Subunit A, which forms intimate contacts with Subunit B, and the position of the CWN1 are indicated. Intermolecular crosslinks are in magenta, and intramolecular crosslinks are yellow. Crosslink 1 links WNK3/A/K159 to B/K218 (WNK1 A/K233 to B/K292), 2 links WNK3/B/K192 to A/K307 (WNK1/B/K266 to A/K381), and intrachain crosslink 3 links WNK3/A/K159 to A/K307 (WNK1/A/K233 to A/K381). Crosslink 4 is not shown. (D) Selected crosslinks observed in the presence of 190 kPa mapped onto a monomer of uWNK1. Residues crosslinked are indicated in Table 2.