Plasmid	Source	internal Lab ID	Used in
pEGFP-BAF	this study	PLA1089	Figure 3, C and E
pEGFP-C1	Clontech, Takara	PLA240, PLA997	Figures 4, E–G, 7, Supplemental Figure 7, B–E
pEGFP-HIS-BAF	T. Haraguchi (Shimi et al., 2004)	PLA1080	Cloning of PLA1089
p-EGFP-IBB	D. Gerlich (Schmitz et al., 2010)	PLA1061	Figure 4C
p-EGFP-KDEL	A. Helenius (IBC, ETH Zurich, Switzerland)	PLA936	Figure 2B
pEGFP-LEM-NES	this study	PLA1098	Figure 7, Supplemental Figure 7, B–E
pEGFP-N1	Clontech, Takara, USA	PLA328	Cloning of PLA1098
pEGFP-N3-RCC1	Y. Zheng (Li et al., 2003)	PLA1074	Figure 4D
p-EGFP-POM121	J. Ellenberg (Beaudouin et al., 2002)	PLA1071	Figure 4B
pIRESpuro2-FLAG-mCherry-LacI	this study	PLA1107	Figures 4, E–G, 5
pIRESpuro2-FLAG-LacI-NLS-mCherry	Y. Barral (IBC, ETH Zurich, Switzerland)	PLA976	generation of stable cell line HeLa LacI-NLS-mCherry
pLacI-NLS-mEGFP	(Wang et al., 2016)	PLA978	Figure 6
pLacO	S. M. Gasser, (Rohner et al., 2008), as in (Wang et al., 2016)	PLA977	Figures 1–9, Supplemental Figures 1–8
p-mCherry-Sec61β	T. Kirchhausen; (Lu et al., 2009)	PLA948	Figures 2A, 8C
p-mCherry-N1 without multiple cloning site	modified from Clontech, Takara, USA		Cloning of PLA1036
pMLBAD (pControl2)	A. Nägeli (Lefebre and Valvano, 2002)	PLA1069	Supplemental Figure 5, D–F
pSR9vector-CMV-mCherry (pControl1)	this study	PLA1036	Supplemental Figure 5, D–F