Fig. 2.

MiR-4649-5p reduces growth and proliferation of TNBC cell lines. A The effect of transient miR-4649-5p mimic transfection on cell growth was assessed by WST-1 assays in three TNBC cell lines (n = 6; mean ± SD; *p ≤ 0.05, ***p ≤ 0.005). B SUM159 and MDA-MB-231 cells were transiently transfected with miR-4649-5p mimic or mimic control and seeded at low density to observe colony formation after 7 (SUM159) or 14 days (MDA-MB-231). Absolute numbers of colonies were counted (as seen in the representative images on the right) and are presented as mean ± SD (on the left) (n = 3; **p ≤ 0.01, ***p ≤ 0.005). C Cell proliferation was assessed 72 h after transient miR-4649-5p mimic transfection in a flow cytometric EdU assay (n = 3; mean ± SD; *p ≤ 0.05, **p ≤ 0.01)