Table 2.
Sensitivity analyses of serum iron biomarkers in 2021 datasets with epigenetic aging accelerations
| Exposure | Outcome | Weighted median | MR-egger regression | Heterogeneitya | MR-PRESSO outlier detectb | Pleiotropyc | |||
|---|---|---|---|---|---|---|---|---|---|
| Beta (95% CI) | P Value | Beta (95% CI) | P value | Beta (95% CI) | P Value | ||||
| Ferritin | Grim | − 0.17 (− 0.57, 0.22) | 3.86E−01 | 0.33 (− 0.26, 0.92) | 2.77E−01 | I2 = 30.9%; Cochrane Q = 101; P = 0.008 | No significant outliers | Intercept = -0.010; P = 0.374 | |
| Ferritin | Hannum | − 0.08 (− 0.45, 0.30) | 6.85E−01 | 0.35 (− 0.17, 0.87) | 1.95E−01 | I2 = 17%; Cochrane Q = 84; P = 0.116 | No significant outliers | Intercept = -0.012; P = 0.217 | |
| Ferritin | IE | 0.07 (− 0.35, 0.49) | 7.53E−01 | 0.16 (− 0.43, 0.76) | 5.91E−01 | I2 = 30.7%; Cochrane Q = 101; P = 0.009 | -0.02 (-0.31, 0.27) | 8.88E−01 | Intercept = -0.003; P = 0.821 |
| Ferritin | Pheno | 0.24 (− 0.27, 0.75) | 3.58E−01 | 0.70 (0.02, 1.39) | 4.90E−02 | I2 = 17%; Cochrane Q = 84; P = 0.117 | No significant outliers | Intercept = -0.011; P = 0.418 | |
| Iron | Grim | 0.53 (0.18, 0.87) | 2.82E−03 | 0.45 (0.06, 0.84) | 3.01E−02 | I2 = 0%; Cochrane Q = 28; P = 0.743 | No significant outliers | Intercept = -0.011; P = 0.374 | |
| Iron | Hannum | 0.47 (0.12, 0.81) | 7.57E−03 | 0.55 (0.18, 0.93) | 6.71E−03 | I2 = 0%; Cochrane Q = 28; P = 0.75 | No significant outliers | Intercept = -0.019; P = 0.125 | |
| Iron | IE | 0.67 (0.29, 1.04) | 5.01E−04 | 0.75 (0.30, 1.21) | 2.79E−03 | I2 = 29.5%; Cochrane Q = 47; P = 0.056 | No significant outliers | Intercept = -0.021; P = 0.159 | |
| Iron | Pheno | 0.24 (− 0.21, 0.68) | 2.93E−01 | 0.45 (− 0.16, 1.07) | 1.60E−01 | I2 = 35.8%; Cochrane Q = 53; P = 0.02 | 0.36 (0.03, 0.69) | 3.96E−02 | Intercept = -0.012; P = 0.534 |
| Iron binding capacity | Grim | − 0.09 (− 0.32, 0.15) | 4.71E−01 | 0.05 (− 0.25, 0.34) | 7.66E−01 | I2 = 27.8%; Cochrane Q = 78; P = 0.03 | No significant outliers | Intercept = -0.007; P = 0.553 | |
| Iron binding capacity | Hannum | 0.01 (− 0.20, 0.22) | 9.18E−01 | − 0.11 (− 0.37, 0.14) | 3.94E−01 | I2 = 6.6%; Cochrane Q = 60; P = 0.333 | No significant outliers | Intercept = 0.001; P = 0.951 | |
| Iron binding capacity | IE | − 0.02 (− 0.28, 0.23) | 8.58E−01 | − 0.15 (− 0.49, 0.18) | 3.72E−01 | I2 = 42.3%; Cochrane Q = 97; P = 0.001 | No significant outliers | Intercept = 0.009; P = 0.501 | |
| Iron binding capacity | Pheno | − 0.17 (− 0.44, 0.10) | 2.19E−01 | − 0.06 (− 0.43, 0.30) | 7.29E−01 | I2 = 22.5%; Cochrane Q = 72; P = 0.071 | No significant outliers | Intercept = 0.005; P = 0.728 | |
| Transferrin saturation | Grim | 0.24 (− 0.01, 0.49) | 6.08E−02 | 0.18 (− 0.13, 0.49) | 2.65E−01 | I2 = 0%; Cochrane Q = 38; P = 0.547 | No significant outliers | Intercept = -0.001; P = 0.909 | |
| Transferrin saturation | Hannum | 0.26 (0.01, 0.52) | 4.38E−02 | 0.29 (− 0.02, 0.59) | 7.63E−02 | I2 = 0%; Cochrane Q = 37; P = 0.626 | No significant outliers | Intercept = -0.004; P = 0.700 | |
| Transferrin saturation | IE | 0.22 (− 0.04, 0.47) | 9.78E−02 | 0.35 (− 0.03, 0.73) | 7.80E−02 | I2 = 29.4%; Cochrane Q = 57; P = 0.042 | No significant outliers | Intercept = -0.009; P = 0.490 | |
| Transferrin saturation | Pheno | 0.38 (0.03, 0.73) | 3.13E−02 | 0.64 (0.11, 1.17) | 2.31E−02 | I2 = 45.1%; Cochrane Q = 73; P = 0.001 | 0.38 (0.11, 0.65) | 9.17E−03 | Intercept = -0.025; P = 0.183 |
aHeterogeneity in the random effect IVW methods was reported
bMR-PRESSO (NbDistribution = 10,000, P < 0.05)
cMR-Egger was used to detect pleiotropy. There is no pleiotropy was observed among all analyses (P > 0.05)
CI, confidence interval, MR-PRESSO, Mendelian Randomization Pleiotropy RESidual Sum and Outlier, IE Intrinsic epigenetic