TABLE 1.
Bacterial strains and plasmids used or studied in this work investigation
| Strain, plasmid, or phage | Description, characteristics, or genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| RZ1032 | Host for pBluescript; HfrKL16 PO/45 [lysA(61-62)] dut-1 ung-1 thi-1 relA1 Zbd-279::Tn10, supE44 | 25 |
| XL-1 Blue | Host for pBluescript with mutated β-lactamase; recA1 endA1 gyrA96 thi hsdR17 (rk−, mk+) supE44 relA1 λ−, lac mutant [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | 8 |
| K-12 J53/R111 | Amoxicillin-resistant strain producing TEM-1 | 27 |
| P9 | Clavulanate-resistant clinical strain producing IRT-6 | 12 and this work |
| P11, P12, P30 | Clavulanate-resistant clinical strains producing IRT-5 (P30), IRT-7 (P11), and IRT-8 (P12) | Cochin Hospital, Paris, France (this work) |
| Plasmids | ||
| pBluescript II KS(−) (pBSKS) | E. coli high-copy-number cloning vector; Aprori ColE1, ori M13 phage | GenBank accession no. X52329 |
| pMBS276D | High-copy-number recombinant plasmid encoding bla gene with Asp276 variation | This work |
| Phage R408 | ||
| M13 helper phage for packaging Bluescript M13 vectors | 35 |