Fig. 1.

Consumption of a high AGE diet results in disorganization of the mouse mammary ductal tree. A Schematic representation of experimental design for examining the effect of AGEs upon mammary gland development. B Representative images of carmine stained whole mounts from 7- B and 12- C week old mice fed regular, low AGE or high AGE diet. D Quantification of ductal extension beyond the central lymph node in 7-week old mice. E Quantification of branch points in 7- and 12-week old mice. Scale bar = 2 mm (B) and 50 µM (C). Mouse numbers: 7 week—regular (n = 4), low AGE (n = 5), high AGE (n = 5); 12 week—regular (n = 4), low AGE (n = 4), high AGE (n = 4). Values are mean ± SEM. ***P < 0.05